课件：GCMS原理与结构.ppt

I show the area repeatability of each pesticides. CV value of all compounds are several %. The result with good linearity and repeatability was obtained. From these results the NCI method is able to use for a quantitative analysis. Another acquisition mode is SIM (selected ion monitoring). In this mode, the voltage is not continuously varied, but is set at only desired mass numbers. This mode is used primarily in quantitative analysis. We can get higher sensitivity than mass chromatogram of scan mode because of longer period measurement for remarkable masses Selection of the mass of target compound is very important with SIM method. High sensitivity analysis is possible depending on the ion selected. （時間があればS/N) I’ve been talking about GCMS, but there’s no limit for this theme. But I think I gave you essentials for GCMS, So I’d like to end my presentation. Here I’ll explain about mass and mass number. Mass of an atom is represented by units that define carbon twelve as 12. We call this unit “ atomic mass unit “ and is denoted by “amu” or “u” Using this unit, mass of a hydrogen atom is 1amu. Other examples are shown in this table. You know that these values are much close to integer. So we call the nearest integer of mass “ mass number” You can easily calculate the mass number and mass of a compound. The example of water molecule is shown here PFTBA relative m/z intensity 69 100.0 131 26.0 219 32.0 414 2.0 502 2.0 614 0.4 CF3 -CF2 -CF2 -CF2 -N -C4F9 C4F9 MS自动调谐流程 寻找质量峰 粗调EM电压和峰宽 调节离子源部件使502达到最佳 调节EM和峰宽达到最佳 质量轴校正 GC-MS常用术语 基峰：179 分子离子：304 质荷比（m/z) 质谱图（棒图） GC-MS常用术语 总离子流图（TIC) TIC和MC（质量色谱图） GC-MS常用术语 总离子流图（TIC) 数据采集模式 Scan（扫描） SIM (选择离子) mass number mass number Time Time [Scan] [SIM] (定性) (定量) Scan 模式（定性） m/z Retention time TIC : Scan 72 54 152 MS 质谱图 SIM 模式（定量） Retention time m/z=72 m/z=152 m/z=54 灵敏度高于 Scan模式 SIM (选择离子模式） SIM主要用于定量分析 只有特定质量数的离子被检测 根据目标化合物选择合适的检测离子相当重要