03_12 extraction of algal lipids英文文献资料.pdfVIP

J Am Oil Chem Soc (2012) 89:1371–1381 DOI 10.1007/s11746-012-2044-8 ORIGINAL PAPER Extraction of Algal Lipids and Their Analysis by HPLC and Mass Spectrometry Jessica Jones • Schonna Manning • Morela Montoya • Karin Keller • Martin Poenie Received: 14 May 2011 / Revised: 23 February 2012 / Accepted: 23 February 2012 / Published online: 13 March 2012 AOCS 2012 Abstract Algae are a promising source of biofuel but different chain lengths. These algal HC could represent an claims about their lipid content can be ambiguous because alternative biofuel to triacylglycerols (TAG) that could extraction methods vary and lipid quantitation often does feed directly into the current petroleum infrastructure. not distinguish between particular lipid classes. Here we compared methods for the extraction of algal lipids and Keywords Algae Biodiesel Biofuels Lipids ELSD showed that 2-ethoxyethanol (2-EE) provides superior lipid HPLC/MS GC/MS Lipid extraction Lipidomics recovery ([150–200 %) compared to other common extraction solvents such as chloroform:methanol or hexane. Abbreviations Extractions of wet and dry algal biomass showed that 2-EE APCI Atmospheric pressure chemical ionization was more effective at extracting lipids from wet rather than BC b-Carotene dried algal pellets. To analyze lipid content we used nor- CE Cholesteryl ester mal-phase HPLC with parallel quantitation by an evapo- CHL Chlorophylls chlorophyll catabolites rative light scattering detector and a mass spectrometer. DAG Diacylglycerol Analysis of crude lipid extracts showed that all major lipid DGDG Digalactosyldiacylglycerol classes could be identified and quantified and revealed a DMF Dimethylformam