Using patterned supported lipid membranes英文文献资料.pdfVIP

protocol Using patterned supported lipid membranes to investigate the role of receptor organization in intercellular signaling Pradeep M Nair1–3, Khalid Salaita4,5, Rebecca S Petit1–3 Jay T Groves1–3,6 1 2 Department of Chemistry, Howard Hughes Medical Institute, University of California, Berkeley, California, USA. Lawrence Berkeley National Laboratory, Physical 3 4 Biosciences Division, Berkeley, California, USA. Lawrence Berkeley National Laboratory, Materials Sciences Division, Berkeley, California, USA. Department 5 6 of Chemistry, Emory University, Atlanta, Georgia, USA. Winship Cancer Institute, Emory University, Atlanta, Georgia, USA. Research Center of Excellence in Mechanobiology, National University of Singapore, Singapore, Singapore. Correspondence should be addressed to J.T.G. (jtgroves@). Published online 31 March 2011; doi:10.1038/nprot.2011.302 physical inputs, both internal and external to a cell, can directly alter the spatial organization of cell surface receptors and their associated functions. Here we describe a protocol that combines solid-state nanolithography and supported lipid membrane techniques to trigger and manipulate specific receptors on the surface of living cells and to develop an understanding of the interplay between spatial organization and receptor function. While existing protein-patternin