高分子传感材料.ppt

生物传感器与生物芯片 (Biosensors and biochips) 3.5 生物传感器与生物芯片 (Biosensors and biochips) 3.5 生物传感器与生物芯片 (Biosensors and biochips) 3.5 生物传感器与生物芯片 (Biosensors and biochips) 3.5 生物传感器与生物芯片 (Biosensors and biochips) 3.5 高分子传感材料—六 Fluorescent DNA-based enzyme sensors Molecular Engineering of DNA: Molecular Beacons Biological Applications of Molecular Beacons 1 分子信标的工作原理是什么？ Molecular Engineering of DNA: Molecular Beacons 分子信标 (Molecular Beacons) 的基本构成是怎样的？ 2 ? 分子信标设计的基本原则有哪些？ 3 Biological Applications of Molecular Beacons ? 1 单核酸多态性与基因筛选 (SNP and Genetic Screening) 2 探测三股DNA (Detection of triplex DNA) 实时检测PCR过程 (Real-time PCR assays) 3 监测蛋白质(Monitoring proteins) 4 生物传感器与生物芯片 (Biosensors and biochips)...... 5 监测蛋白质(Monitoring proteins) 3.4 Application of MBs in enzymatic studies. a) Real-time monitoring of SSB–DNA binding: The MB binds to the SSB protein whereby its structure is disrupted and its fluorescence is restored. b) Detection of the enzymatic digestion of DNA: The enzyme cleaves the MB and destroys the hairpin structure to restore fluorescence. c) Detection of LDH–DNA interactions: LDH binds the MB and disturbs its structure, whereby fluorescence is enhanced. 监测蛋白质(Monitoring proteins) 3.4 监测蛋白质(Monitoring proteins) 3.4 监测蛋白质(Monitoring proteins) 3.4 监测蛋白质(Monitoring proteins) 3.4 The interactions between two key macromolecular species, nucleic acids and proteins, control many important biological processes. There have been limited effective methodologies to study these interactions in real time. In this work, we have applied a newly developed molecular beacon (MB) DNA probe for the analysis of an enzyme, lactate dehydrogenase (LDH), and for the investigation of its properties of binding with single-stranded DNA. Molecular beacons are single-stranded oligonucleotide probes designed to report the presence of specific complementary nucleic acids by fluorescence detection. The interaction between LDH and MB has resulted in a significant fluorescence signal enhancement, which