单增李斯特菌表面增强拉曼光谱检测技术研究-食品科学专业毕业论文.docx

ABSTRACTL豇fe一口，行D力D￡Ⅳ艮增珊芒墨can ABSTRACT L豇fe一口，行D力D￡Ⅳ艮增珊芒墨can canse bactefial food poisDni王19 by meatdaily products and other．It is hanll如lto h【man health a11d poses aIl importallt tllI·eat to public sec嘶咄 The deVelopment of 缸t a11d reliable sensing techiques to detect food勘me mlcroo唱aIlisms is an indispensable key to food industry a11d healmcare．IIl recent years， s时．ace e11llaIlced R姗a11 spectroscopy haS be锄widely used in a鲥cultllre，food indust姒 ch锄isny indust珥A standard protoc01、】l郴established for detection of南od-bome microo唱arIisms by R锄a11 spectroscopic tectulique． Before the experiment，aH the tested s仃ains were identified accurately based on tlleir 1 6SrDNA gene sequences．ARer perfloming HCA we detemlined SERS had su伍cient res01ution to di任．erentiate closely related ba曲Bria within a genus掣own on s01id锄d liquid medium．Unsupefvised PCA a11d HCA models and supeⅣis甜DA-based chemometric models were used to dia’erentiate four common food．bome microorgaIlisms 【印砂，ococc凇strain，E∞疗type strain，勋砌。忍e如type strain and三括把，l胁strains) between R锄aIl spectra of Gr姗-positive and Gr锄-negative bact丽a，at also dif]FereIl石ates bactena at genus and Species leVel． 111en， we evaluated R锄aIl spectroscopy in comblnatIon wl廿1 ch锄ome啊c analysis to identi矽six cIoseIy related species witmn the Llst酣a genus(including the humall pathogen￡西饱砌m册ocyfp嚣甩嚣)舶m broth and directly 行om milk． Here， we also used panial least．squares re掣ession model to dls嘶minate aIld quanti母the acmal concentration of a specific Listeria s仃ain in a bacterial mixture．The results Show that，culture conditions had greater innuence on the R锄an spectroscopy of bacte订a；R锄aIl spectrosc叩y in combination with DA-based ch锄ometric model(Distallce dis嘶minaIlt analysis，97．4％；aIld Bayes dis谢minant analysis，98．8％)caIl claLssified four common food’bome microo唱anisms at tl矾e levels；R锄aIl spectroscopy in combination with ch锄ometric analysis to identi黟six cIosely related species winlin tIle Listeria genus(incIuding me h