核供体细胞体外培养与其对猪克隆胚生产效率的影响.pdfVIP

ABSTRACT CUl月UREoFNUCLEARDONoRCELLSAND INVITRO THEPRODUCTION ITSEFFECTON OFCLONED EMBRYOSINSWINE Abstract cellnuclear and in Somatic transfer(SCNT)behavedhugepotentialprospectgood domesticanimal animal experimental quality breeding，homogeneous production, mammalian endangered speciesprotection,therapeutical animal andSO kindsofsomaticcellswereusedasnuclear forth．Currently,many production cellsto clonedanimals．ClonedSCNThavesome donor produce pigsby advantages to beusedasmodelsofhumandiseasesanddonor for potentially organs havebeenthefocusintheSCNT some xenotransplantation,and area．Althoughprogress havebeenmadeto clone SCNT stillexisted yield piglets诵tlltechnique，theremany was withlessthanl％of total ofclone stilllow efficiencypigproduction problems，e．g．the clonedanimalshaveabnormal transferredsurvivaltothetermandthe may embryos iScriticalforthesuccessofcloneanimal eellselection production，and development．Donor itisalsoan factor the was cloningefficiency．Thepresentstudy importantimpacting theeffec