利用大孔微载体体外制备工程化组织.pdf

生 物 工 程 学 报 Chin J Biotech 2008, January 25; 24(1): 74-82 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ ©2008 Institute of Microbiology, CAS CSM, All rights reserved. 研究报告 汤 强, 周 燕, 陈 飞, 谭文松 , 200237 : 组织工程从诞生至今已有二十年的历史, 微载体在组织工程领域的应用研究近年来才日趋升温。利用大孔微载 体Cytopore 体外构建工程化微构组织, 并基于其聚团特性, 进行灌注再装配大组织的尝试。结果20 d 内微构组织达最 7 3 高细胞密度16.4×10 cells/cm ; 32 d 后, 所形成的微构组织整体均质性好、活性高、基质生成丰富; 培养后期微构组织 发生明显聚团, 经灌注再装配得到细胞和基质分布均匀的厘米级(12 mm × 6 mm) 大组织。说明利用大孔微载体构建工程 化组织是一种行之有效、富有潜力的体外组织构建方案, 有望构建尺寸≥5 mm 、细胞和基质分布均匀的大组织。为体 外组织构建提供新的思路。 : 大孔微载体, 微构组织, 聚团, 大组织 Preparing Engineered Tissues in vitro by Macroporous Microcarriers Qiang Tang, Yan Zhou, Fei Chen, and Wensong Tan State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China Abstract: Macroporous microcarriers (Cytopore) were used to construct microfabricated tissues. Perfusion approach was used to reassemble macrotissue on the basis of their aggregative property. Microfabricated tissues reached the maximum cell density with 7 3 16.4×10 cells/cm after 20 days. They were uniform with high cell viability and massive matrix as a whole after 32 days. The aggre- gation of the microfabricated tissues was visible especially in the later period of the culture, and a centimetre-size(12 mm × 6 mm)macrotissue with uniform cell and matrix distribution was obtained through perfusion reassembly. Constructing engineered tissues us