DnaK蛋白质的纯化及其生物化学性质分析-食品萝科学专业毕业论文.docxVIP

ABSTRACTHeat ABSTRACT Heat Shock Protein 70kDa，Hsp70s，are a class of highly conseⅣed m01ecular chaperone WIlich are essential for maintaining cellular protein homeostasis in orgaIlisms ranging f．rom bacteria to m猢a1． DnaK，a major Hsp70 in E cD，f，has been widely used as a paradigm for studying Hsp70s．nle latest s01Ved c呵stal灿cture of Dn水in complex wi也ATP shows t11ere are two m01ecules of DnaK—AIP in me asymmetric u11it．Interestingly，谢m seVeral hydrogen bonds t11e interface between tlle two molecules of DnaK are 1a玛e witll good sul．|’ace complementaIi够，suggesting functional importallce of mis cr