e8微生物学-南京师范大学英文版PowerPoint课件.pptVIP

Main topics: BREEDING BY INDUCED MUTATION BREAKTHROUGHS LEADING TO RECOMBINANT DNA TECHNOLOGY STRATEGIES OF USING RECOMBINANT DNA TECHNIQUES PRODUCTIONS OF RECOMBINANT PROTEINS GENETIC MODIFICATION OF ORGANISMS Advantages in production of recombinant protein i. Use of modified gene; ii. Increase of gene copies; iii. Strong promoter for transcription; iv. Optimized translation; v. Optional regulation; vi. Easy cultivation. A easy way to modify a gene YYY XXX YYYYYY XXXXXX YYYAAATTT CCCAAAYYY XXXTTTAAA GGGTTTXXX Blunting Kination Ligation PCR i. Use of modified gene * * MICROBIOLOGY Chapter 8. Microbial Genetics by Weilan Shao Definition review Genetics is the science of heredity; it is concerned with the physical and chemical properties of the hereditary material, how this material is transmitted from one generation to the next, and how the information it contains is expressed during the development of an individual. ---- Stanley R Maloy et al., in MICROBIAL GENETICS, 1994 MICROBIOLOGY Chapter 8. Microbial Genetics IV. Applications of Microbial Genetics Main topics: BREEDING BY INDUCED MUTATION BREAKTHROUGHS LEADING TO RECOMBINANT DNA TECHNOLOGY STRATEGIES OF USING RECOMBINANT DNA TECHNIQUES PRODUCTIONS OF RECOMBINANT PROTEINS GENETIC MODIFICATION OF ORGANISMS Main topics: BREEDING BY INDUCED MUTATION BREAKTHROUGHS LEADING TO RECOMBINANT DNA TECHNOLOGY STRATEGIES OF USING RECOMBINANT DNA TECHNIQUES PRODUCTIONS OF RECOMBINANT PROTEINS GENETIC MODIFICATION OF ORGANISMS A “classical” genetic technique for strain breeding Physical agents Mutagenic agents e.g. X-rays, g-rays, UV Chemical mutagens e.g. base analogs, nitrous acid, alkylating, arylating agents UVL VL Photolyase UV Damage photoreactivation of DNA EXAMPLE Induced mutation of Penicillium chrysogenum Breeding of strain NRRL-B25 for production of penicillin Strain Mutagen Yie