蛋白质相互作用专题课件.pptx

蛋白质相互作用Protein interaction：two or more proteins (same or different) interact or form complexMultiple protein interaction: e.g. F-actin, microtuble, proteins interact to form a functional structureEnzyme complex: e.g. fatty acid synthase, multiple enzymes to catalyze a set of reactionsRibosome: protein complex to execute a complicated reactionAntibody-antigen interaction: to neutralize pathogenKinase-substrate interaction: to pass signalTranscription factors: to induce gene expression etc……Protein: an important constituent of lifeProtein interaction: make the difference of being live or dead.Protein interaction:1、protein interaction has its own biological functions and significance. It is part of the life. It is important for life itself. We study it to understand life. e.g. the mechanism of Gal 4 in regulating gene expression2、protein interaction provides the technology useful for us to dissect and understand life. It is useful tool. We use it to study life. e.g. yeast two-hybridization: transcription factor Gal 4 interact with DNA and polymerase complex Two-hybridization is not what yeast has in mind when it creates the Gal 4 transcription factor.To understand the functions and importance of protein interaction in biological processProtein interactions are overly complicated. Think about 10000 proteins and each capable of interacting with hundreds if not thousands of others. Plus all the modifications.Then, where to get started：1、starting from an important protein or a protein that can get you a publication2、mining the database to identify something important interactionsData mining: try to figure out something useful from chaosData mining is tricky. You may fish out a diamond or you may step on a mine. Work hard. Diamond or mine, it is your luck.Analysis of important proteins: most important ones are picked. You don’t know beforehand if the new protein you pick is an important one. 1、Starting from an important protein or evente.g. EGFR endocytosisReceptor clust