整体原位杂交检测ER基因在小鼠胚胎发育中的表达-动物学杂志.pdf

Chinese Journal of Zoology 2008, 43( 3) : 39~ 44 ER ! * 张子峰 樊少华 陆 军 吴冬梅 单 群 胡 斌 郑元林 ( 221116; 221116; ! 210000) : ( ER) RNA , ER RTPCR, ER, ERpGEM3Z , Hind ∀EcoR# DNA , T7 Sp6 RNA , ERERpGEM3Z , di ERRNA ER 105 dpc , 135 dpc , ER : ( ER) ; RNA ; ; : Q812 : A : 2008) 033906 Expression of Estrogen Receptorin Mouse Embryo as Revealed by Whole Mount in Situ Hybridization ! ZHANG ZiFen FAN ShaoHua LU Jun * WU Don Mei SHAN Qun HU Bin ZHENG YuanLin ( School of Lif e Science, Xuzhou Normal University , Xuzhou 221116; Key Laboratory of iotechnologyf or Medicinal Plants of Jiangsu Province, Xuzhou 221116; ! School of asic Medical Science, Southeast University, Nanj ing 210000, China) Abstract: Objective: In order to study the expression of estro en receptor ( ER) in mouse embryo, the sense and anti sense RNA probes for hybridization of ERwere prepared. Methods: The ERfra ment which was obtained by RTPCR was subcloned into plasmids pGEM3Z, and then the recombinant plasmids ERpGEM3Z were linearized with the restriction enzymes, Hind ∀ and EcoR# . Usin T7 and Sp6 RNA polymerase, respectively, the di oxi enin ( di ) labeled sense and antisense probes were transcribed in vitro. Then the expression of ERin mouse embryo was examined with the probes by whole mount in situ hybridization. Results:The ene fra ment of ERwas cloned and the cRNA probes were prepared with di oxi eninlabeled. ER ene was expressed in the forebrain, s