Affinity purification of antibody抗体亲和纯化.docVIP

Affinity purification of antibody Step 1. Chemically cross-link antigen to beads for affinity purification We use HiTrap NHS-activated HP column for affinity antibody purification. This is a pre-packed column of N-hydroxy-succinimide (NHS) cross linked to HP Sepharose beads. NHS reacts with ligands containing amino groups to give a very stable amide linkage. This column can be connected to FPLC. It comes in 100% isopropanol and once it is exposed to water-based solution, NHS quickly loses its activity so that antigen has to be loaded immediately after the column is exposed to water. Material