苦瓜种质遗传多样性的SRAP标记分析-IngentaConnect.pdfVIP

植物遗传资源学报2013,14(1): Journal of Plant Genetic Resources 苦瓜种质遗传多样性的SRAP标记分析 1,2 2 2 赵秀娟 ,宋建文 ,胡开林 1 2 ( 广东科贸职业学院,广州510430;华南农业大学园艺学院,广州510640) 　 　 摘要:从144 对SRAP 引物中筛选出61对多态性强、重复性好的SRAP 引物,对43份苦瓜种质DNA进行了扩增,共得到 2078条谱带,其中多态性谱带863条,平均每对引物扩增得到14.15 条多态性谱带,多态性位点百分率为42.11%。 用UPM- GA方法进行聚类分析,供试苦瓜种质的遗传相似系数为0.50~0.95,在阈值0.65 处(L1)可将苦瓜分为2 个类群,第Ⅰ类群 为野生种,第Ⅱ类群为半栽培种和栽培种;在阈值0.80 处(L2)将第Ⅱ类群分为5 个亚类群,L2 的划分反映了苦瓜种质间的遗 传多样性与果实性状、来源或地理分布有较高相关性;在阈值0.83 处,第Ⅱ1亚类群又分为4 类,大致分为滑身苦瓜(粗棱无 瘤)、大顶苦瓜(棱细大圆瘤、倒锥形)、棱细圆瘤苦瓜以及尖刺瘤或凸瘤苦瓜,分类结果与苦瓜的刺瘤有无、棱条粗细、瘤的形 状和瓜色等密切相关,初步认为刺瘤苦瓜为较原始类型。 关键词:苦瓜;种质资源;遗传多样性;SRAP Genetic Diversity Analysis of Bitter Gourd Germplasm Resources Based on SRAP Markers 1,2 2 2 ZHAOXiu-juan ,SONGJian-wen ,HU Kai-lin 1 ( Guangdong Science and Tradition College of Vocation,,Guangzhou510430; 2 College of Horticulture,South ChinaAgricultural University,Guangzhou510640) Abstract:61pairs of SRAP primerswith rich polymorphism and goodrepeatability were chosenfrom 144 test- ed pairs and used to amplify genomic DNA of 43 accessions of bitter gourds.A total of 2078 amplicons were ob- tained of which 863(42.11%)were polymorphic,with an average of 14. 15 polymorphisms observed per primer pair.Cluster analysis was conducted using UPMGA method.It was concluded that the genetic similarity among 43 bitter gourd germplasmsrangedfrom0.50 to0.95.All accessionswere clustered broadly into two groupsat the simi- larity coefficient of0.65.The first group included wild accessions and the second group consisted of semi-cultivated andcultivatedaccessions.Thesecondgroupw