海陆渐渗系棉花吐絮期叶绿素含量、荧光参数及相关性状的QTL定位分析.pdfVIP

棉 花 学 报 Cotton Science 20 15袁27 渊5 冤院4 17―426 陆渐渗系棉花吐絮期叶绿素含量 荧光参数及 相关性状的QTL 定位分析 福喜 汤丽魁 唐媛媛 李志博 赵瑞海 雷军 魏亦农* ( 河子大学农学院/ 新疆兵团绿洲生态农业重点实验室 新疆 河子832003) 摘要院以海陆渐渗系13-1 辽棉12 组配的195 个单株的F2 群体为作图群体 利用SSR渊Simple sequence repeat) 标记和JoinMap3.0 软件构建遗传连锁图谱 构建的遗传连锁图谱包含39 个多态性标记尧13 个连锁群 该图谱 总长1174.4 cM 覆盖棉花基因组的26.7% 利用IciMapping 完备区间作图法对F2:3 家系进行相关性状的QTL 定位 共检测到30 个叶绿素荧光参数尧7 个叶片干物质含量尧6 个叶面积指数尧1 个叶绿素含量的QTL 位点 分 布在8 条染色体上 在同一染色体共标记区间内存在多个性状的QTL 部分位点加性遗传效应来自同一亲本 与干物质含量尧最大光化学效应相关的QTL 位点在3 条染色体上不同标记区间内重复出现 与叶面积指数尧 最大光化学效应相关的QTL 位点在4 条染色体上不同标记区间内重复出现 表现出遗传上的一因多效或基 因连锁效应 可用于高光效聚合育种遥 关键词院棉花曰海陆渐渗系曰叶绿素荧光参数曰SSR 标记曰QTL 定位 中图分类号院S562.035 文献标志码院A 文章编号院1002-7807 渊20 15冤05-04 17-10 10.11963/issn.1002-7807.201505005 Rong Fuxi, Tang Likui, Tang Yuanyuan, Li Zhibo, Zhao Ruihai, Lei Jun, Wei Yinong* ( g 832003, ) In this study, a genetic linkage map was constructed for an F2 population of 195 cotton plants derived from the cross between the parental introgression lines 13-1 and Liao 12 with SSR (simple sequence repeats) markers using the JoinMap 3.0 software. The map included 39 SSR loci and 13 linkage groups, covering 1174.4 cM and accounting for 26.7% of the cotton genome. Based on the inclusive composite interval mapping method with IciMapping, 44 QTLs (quantitative trait locus) were i- dentified on eight chromosomes in the F2:3 population, including 30 QTLs for chlorophyll fluorescence parameters, seven for leaf dry material content, six for leaf area index, and one for chlorophyll content. Multiple trait QTLs were located between the com- mon range of markers on the same chromosome and the additive effect of some QTLs were inherited from the same parent. Some QTLs related to dry