第一次课现代生物技术综合大实验.pptVIP

Transforms with high efficiency. Like many cloning strains, DH5 alpha has several features that make it useful for recombinant DNA methods. The endA1 mutation inactivates an intracellular endonuclease that degrades plasmid DNA in many miniprep methods[1]. The hsdR17 mutation eliminates the restriction endonuclease of the EcoKI restriction-modification system, so DNA lacking the EcoKI methylation will not be degraded. DNA prepared from hsdR strains that are wt for hsdM will be methylated and can be used to transform wt E. coli K-12 strains. Δ(lacZ)M15 is the alpha acceptor allele needed for