不同产地丹参药材红外光谱分析研究.docVIP

[基金项目] [基金项目]“重大新药创制”科技重大专项（中药生产技术及过程控制技术标准平台2009ZX09308-003） [通讯作者] *龚慕辛，Tel(010 E-mail:gongmuxin@126.com 王凌1，2，龚慕辛1*，王智民3，万楷杨3 （1. 首都医科大学中医药学院，北京 100069 ； 2.中国中医药科技开发交流中心，北京 100027；3.中国中医科学院中药所，北京 100070） 摘要：目的：建立红外光谱技术，快速鉴别丹参药材产地及确定丹酚酸B含量高低。方法：采集来自4个产地的40批丹参药材的红外光谱信息，寻找可以反映产地特征的二阶导数红外光谱特征峰，以及可进行丹酚酸B快速定量的原谱特征峰。结果：不同产地丹参药材的二阶导数红外光谱特征吸收峰存在差异，可以反映丹参药材的不同产地；原谱1262 cm-1峰的吸收峰高基本不受其他成分的影响，可以反映丹酚酸B含量且与液相色谱含量结果呈正相关，实现丹酚酸B的快速半定量。结论：建立的红外光谱技术可以为中成药生产过程中， 关键词：丹参 产地 红外光谱 丹酚酸B The study of Salviae miltiorrhizae radix et rhizoma by Infrared Spectroscopy Wang Ling1。2, Gong Mu-xin1*，Wang Zhi-min3，Wan Kai-yang3 (1. Chinese Medical Institute, Capital Medical University, Beijing 100069, China; 2.National Center of Traditional Chinese Medicines, Beijing 100027, China; 3.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100070, China;) [Abstract] Objective: To establish a fast method to evaluate the geographic origins of salviae miltiorrhizae radix et rhizoma and the contents of salvianolic acid B based on infrared spectroscopy (IR). Methods: IR spectra of forty samples collected from 4 different origins were analyzed to search for the characteristic peaks on second derivative (2D) spectra to reflect the characteristics of origin, also to search for a characteristic peak on normalized spectra for fast quantitation of salvianolic acid B. Results: Some characteristic peaks on 2D spectra were found which reflect 4 different origins. The intensity of 1262 cm-1 on normalized spectra was found to correlate well with the content of salvianolic acid B determined by high performance liquid chromatography, which realize the fast quantitative analysis of salvianolic acid B. Conclusions: The proposed IR method provides a new alternative of fast quality evaluation of salviae miltiorrhizae radix et rhizoma for manufacture [Key words] Salvia miltiorrhiza Bge．; Infrared Spectroscopy; Salvianolic acid B 丹参系唇形科植物丹参Salvia miltiorrhiza Bge.的干燥根及根茎[1]