基因工程作业(引物设计).docVIP

PAGE PAGE 11 基因工程作业 口腔鳞癌组织中肿瘤转移相关基因1（MTA1） 一、选择原因及应用 口腔鳞癌组织中肿瘤转移相关基因1（MTA1）在蛋白和mRNA的表达水平，揭示其与口腔鳞癌（OSCC）发生、发展的关系。方法采用免疫组织化学法和原位杂交技术检测46例OSCC标本、15例口腔黏膜白斑与20例正常口腔黏膜标本中MTAl基因的表达水平，并分析其与OSCC临床病理学参数的关系。结果MTA1蛋白和MTA1mRNA在OSCC组织中的表达水平显著高于口腔黏膜白斑和正常口腔黏膜（P〈0．05），口腔黏膜白斑中MTA1蛋白和MTA1mRNA表达水平显著高于口腔正常黏膜（P〈0．01），MTA1蛋白和MTA1mRNA表达与肿瘤浸润深度和淋巴结转移密切相关（P〈0．05）。结论MTA1基因在蛋白和mRNA的表达水平在OSCC发生、发展及浸润转移过程中起一定促进作用，有望成为判断OSCC预后及选择治疗方案的一个新肿瘤标志物。 2查阅NCBI得到MTA1相关信息并获得目的基因 PREDICTED: Gorilla gorilla gorilla metastasis associated 1 (MTA1), mRNA NCBI Reference Sequence: XM_004055801.1 FASTA Graphics LOCUS XM_004055801 2872 bp mRNA linear PRI 03-DEC-2012 DEFINITION PREDICTED: Gorilla gorilla gorilla metastasis associated 1 (MTA1), mRNA. ACCESSION XM_004055801 VERSION XM_004055801.1 GI:426378238 KEYWORDS . SOURCE Gorilla gorilla gorilla (western lowland gorilla) ORGANISM Gorilla gorilla gorilla Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Gorilla. COMMENT MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence ( NW_004005914) annotated using gene prediction method: GNOMON, supported by mRNA and EST evidence. Also see: Documentation of NCBIs Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gorilla gorilla Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers sour