乳腺癌血清蛋白质组与尿代谢物组协同分析研究.docVIP

基金项目:国家自然科学基金重大研究计划重点资助项目浙江省科技计划项目(2004C33026 作者单位:310003杭州,浙江大学医学院附属第一医院(沈朋;浙江大学药物信息学研究所(曾真、程翼宇电子信箱:shenp@zju. edu.cn ?临床实验诊断研究? 乳腺癌血清蛋白质组与尿代谢物组协同分析研究沈朋　曾真　程翼宇 　　【摘要】　目的　研究血清蛋白质组和尿代谢物组协同分析乳腺癌诊断方法。方法　用双向电 泳、高效液相色谱和液质联用技术对7份正常人与14份乳腺癌患者血清蛋白质组和尿代谢物组进行 分析,从中寻找差异蛋白和代谢组模式差异。结果　与正常人相比,乳腺癌患者血清中谷胱甘肽S2 转移酶M5呈高表达状态,尿中乳清酸核苷、12甲酰化腺苷、S2腺苷2L2蛋氨酸及N22甲酰化鸟苷等4种 核苷代谢物组成模式异常。结论　乳腺癌患者体液内化学物质与正常人相比具有特异性模式差异。 经肿瘤蛋白质组与代谢物组协同研究,有望建立肿瘤早期诊断新方法,进行肿瘤发病机理研究。 【关键词】　乳腺肿瘤;　蛋白质组学;　代谢组学 Stud i es on i n tegra ted ana lysis of seru m proteo m i cs and ur i n ary m et abono m i cs of brea st cancer pa ti en ts　　SHEN Peng3,ZEN G Zhen,CHEN G Y i2yu13The First A ffiliated Hospital,College of M edicine,Zhejiang U niversity,Hangzhou310003,China Em ail:shengp@zju.edu.cn 【Abstract】　O bjecti ve　To study on the diagnosis methodol ogy of breast cancer using integrated analysis of p r oteom ics and metabonom ics app r oach1M ethods　The seru m sa mp les fr om the14breast cancer patients and7healthy peop le were analyzed by t w o2di m ensi onal electr ophoresis in order,t o find differential exp ressed p r oteins1On the other hand,an HP LC2based urinary metabonom ics p r ofiling was e mp l oyed t o p ick out che m ical patterns bet w een breast cancer patients and healthy peop le1Results　Glutathi one S2transferase M5was found highly exp ressed in the breast cancer patients′seru m.The concentrati on pattern of S2 Adenosyl m ethi onine,O r otidine,12M ethyladenosine and N22Methylguanosine in patients′urine were peculiarly different fr om that in the healthy peop le1Conclusi on　There are peculiar che m ical pattern differentiae bet w een the body fluid of breast cancer patients and the body fluid of healthy peop le1Synthetic study of cancer p r oteom ics and metabonom ics can contribute not only t o p r ognosis of cancer but als o t o the pathogenesis of cancer1 【Key words】　B reast neop las m s;　Pr oteom ics;　Metabonom ics 　　基因组学、蛋白质组学和代谢组学等各类“组学”的兴起,已从寻找单一生物标志物转向寻找特异性的一组标志物群(又称“特征模式”,开创了肿瘤诊断方法研究的新途径。目前,蛋白质组