副溶血性弧菌脂肪酸的GC.doc

43株副溶血性弧菌菌体脂肪酸的成分分析? ? 基金项目： 广东省医学科研基金项目(A2013657)。作者简介：郭川，男，主管技师，主要从事病原微生物研究。 郭川1，陈银海1，林洁敏1，陈冠武2，陈婉1 汕头市疾病预防控制中心, 广东汕头 515041；2. 汕头市出入境检验检疫技术中心, 广东汕头 515031；） 摘要：目的：了解副溶血性弧菌菌体脂肪酸组成的特征，为该菌的脂肪酸鉴定与分型提供理论依据。方法：对副溶血性弧菌标准菌株VPL4-90及42株分离株（VP-01~VP-42）进行菌体脂肪酸提取及甲酯化，通过GC-MS对脂肪酸甲酯进行分离、组分鉴定，并对菌株的脂肪酸特征进行分析。结果：所有菌株的提取物气相色谱图上都有7个峰经质谱鉴定为脂肪酸甲酯，它们分别为C14:0、C15:0、C16:1、C16:0、C17:0、C18:1、C18:0脂肪酸的甲酯；其中含量最高的脂肪酸是C16:0，而C16:1/C18:0含量较低，C14:0/C15:0/C17:0含量则最低；聚类分析结果显示当平方欧氏距离≤9时，副溶血性弧菌分为3个群，但该分群无法反映O血清型别上的差异。结论：43株副溶血性弧菌的菌体脂肪酸组成特征相似，含量皆呈现C16:0 C16:1/C18:0 C18:1 C14:0/C15:0/C17:0特征；脂肪酸分型对副溶血性弧菌种内的菌型不具备鉴别能力。 关键词：气相色谱；质谱；副溶血性弧菌；脂肪酸；聚类分析 Analysis on Cellular Fatty Acid Components of 43 Strains of Vibio parahamolylicus Guo Chuan1, Chen Yin-hai1, Lin Jie-min1, Chen Guan-wu2, Chen Wuan1 （1. Center for Disease Control and Prevention of Shantou, Shantou, Guandong 515041, China; 2. Technology center of Shantou entry exit-inspection and Quarantine Bureau, Shantou, Guandong 515031, China） Abstract: Objective To figure out the characteristics of cellular fatty acid from Vibio parahaemolyticus, and provide a theoretical basis for the fatty acid typing and determination of Vibio parahaemolyticus. Method Fatty acid from Vibio parahamolylicus (including standard stain VPL4-90 and 43 isolated strains VP-01~VP-42) were extracted and methyl esterificated. GC-MS was applied to separate and determine the fatty acid methyl ester (FAME). Characteristic of the cellular fatty acid from the strains was subjected to analysis. Result 7 peaks of GC were identified by MS as FAMEs, which were the methyl ester of C14:0, C15:0, C16:1, C16:0, C17:0, C18:1 and C18:0; In the 7 components of cellular fatty acids, C16:0 was the component with highest content, while the C16:1/C18:0 were the components with lower content, and the C14:0/C15:0/C17:0 were the component with lowest content; Result of Cluster Analysis showed that the strains could be divided into 3 groups, while the Squared Euclidean distance≤9. However, the grou