黑龙江省大豆遗传结构及遗传多样性分析.doc

作物学报 ACTA AGRONOMICA SINICA 2009, 35(2: 228?238 ISSN 0496-3490; CODEN TSHPA9 /zwxb/ E -mail: xbzw@ DOI: 10.3724/SP.J.1006.2009.00228 黑龙江省大豆种质遗传结构及遗传多样性分析 秦 君2,3 李英慧1 刘章雄1 栾维江1 闫 哲1 关荣霞1 张孟臣3 常汝镇1 李广敏2,3,* 马峙英2 邱丽娟1,* 1 中国农业科学院作物科学研究所 / 国家农作物基因资源与遗传改良重大科学工程开放实验室/农业部作物种质资源与生物技术重点开放实验室, 北京100081; 2河北农业大学, 河北保定071001; 3河北省农林科学院, 河北石家庄050031 摘 要: 利用22个表型性状和60个微卫星(simple sequence repeat, SSR位点对黑龙江省140份代表性种质(78份地方品种和62份育成品种 进行分析, 根据UPGMA (unweighted pair group method with arithmetic mean和Model-base 对SSR 数据进行遗传结构划分。结果表明, 参试品种可分为2大类群, 第II 类群的各项多样性指标均高于第I 类群, 2个类群遗传距离为0.2427; PCO结果显示这2个类群在不同区域, 这与地理来源和育成年代密切相关。依据品种类型分为育成品种和地方品种两组, 后者的各项多样性指标均高于前者, 两组间的遗传距离为0.1131。依据表型数据的PCO 分析表明, 分布区域与品种类型有关, 与SSR 结构分类的结果吻合度低, 两组品种主要在3个主成分的6个表型性状上有所不同。它们不是2个相对独立的遗传群体, 根据分子标记和表型分类各有特点; 建议在种质遗传多样性研究中将分子数据和表型数据结合起来。 关键词: 黑龙江省; 大豆; 微卫星标记; 遗传结构; 遗传多样性 Genetic Structure and Diversity of Soybean Germplasm in Heilongjiang, China QIN Jun2,3, LI Ying-Hui1, LIU Zhang-Xiong1, LUAN Wei-Jiang1, YAN Zhe1, GUAN Rong-Xia1, ZHANG Meng-Chen3, CHANG Ru-Zhen1, LI Guang-Min2,3,*, MA Zhi-Ying2, and QIU Li-Juan1,* 1 National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Germplasm Biotechnology, Institute of Crop Sci- ences, Chinese Academy of Agricultural Sciences, Beijing 100081, China, 2 Agricultural University of Hebei, Baoding 071000, China; 3 Hebei Aca- demy of Agricultural and Forestry Science, Shijiazhuang 050031, China Abstract: Heilongjiang province is the main production area for soybean (Glycine max in China, having the high genetic diver-sity of cultivated soybean. It is useful for breeding and production in the region to develop new germplasm. The aim of this study was to reveal genetic structure and genetic diversity of spring sowing soybean germplasm from Heilongjiang, and provide a reli-able strategy in soybean breeding program. A total of 140 accessions of soybean cultivars including 78 landraces and 62 deve- loped cultivars were investigated using 60 microsatellit