基于微胶囊生物传感器.pptVIP

基于微胶囊的光学生物传感器Microcapsules as optical biosensors Introduction color-changing particles reside primarily in the dermis respond to changes in interstitial fluid composition measurable shifts in their optical properties. external optical device pass light into the skin collectreflected or emitted light perform analysis to report interstitial composition to the patient in a continuous or spot fashion smart tattoo Examples of this concept : fluorescent microspheres/capsules, hollow fiber membranes encapsulating sensing chemistry, and polymeric slabs doped with fluorescent reagents. the target species are able to freely diffuse into the container, while permanently compartmentalizing the sensing reagents The key to making this approach viable： engineer implantable materials, sensitivity, operational stability, and biocompatibility for long-term use Encapsulation within polyelectrolyte multilayer capsules Nano-engineered microcapsule carriers is an effective means of encapsulating sensing reagents. Different approaches to entrap optical sensing assays: various types of microvesicles, including liposomes, polymersomes, and polyelectrolyte microcapsules. Polyelectrolyte microcapsules are formed by depositing alternating layers of charged polymers onto sacrificial templates using the layer-by-layer (LbL) nanoscale self assembly process, which is followed by the removal of the template via chemical treatment. layer-by-layer (LbL) nanoscale self-assembly process allows for an even wider assortment of candidate materials. The goal of encapsulation is to trap the sensing reagents behind a semi-permeable Barrier. The sensor chemistry must be trapped in sufficient quantity for the optical response to be measured. Three principal methods: Precipitation, adsorption/absorption, diffusion 1. Diffusion 2. adsorption/absorption Advantage: virtually any removable template can be used Disadvantages: low loading efficiency, requirement to use materia