HPLC测定决明子中决明子苷A，B含量.docVIP

???? HPLC测定决明子中决明子苷A，B含量 ???? ????　摘要　目的：建立决明子中降血脂有效成分决明子苷A，B(以下简称苷A，B)的HPLC定量测定方法。方法：决明子药材经氯仿脱脂后再由甲醇提取，甲醇提取液经释释后直接进样分析；在μ-Bondapak C18柱上，以乙腈--水-四氢呋喃-冰醋酸(20∶76.5∶3.0∶0.5)为流动相，流速为1ml.min-1，检测波长为278nm，测定了样品中苷A、B含量，并采用光谱法和色谱法对A、B峰的色谱纯度进行了鉴定。结果：苷A、B的保留时间分别为12.13min和13.27min，经鉴定苷A、B峰均为单一物质色谱峰：苷A、B的标准曲线分别在0.25～1.25μg(r=0.9999)和0.125μg～0.625μg(r=0.9999)之间有较好线性关系，苷A、B的加样回收率分别为(96.83±3.53)%和(97.78±2.16)%。结论：本法专属性强，可用于决明子中决明子苷A、B的含量测定。 　　关键词　决明子苷A、B；决明子；HPLC Analysis of cassiaside A and B in the seeds of Cassia obtusifolia by HPLC Liu Songqing (Liu SQ),Gao Zhentong (Gao ZT),Yang Dajian (Yang DJ),et al (Department of Pharmacy,Southwest Hospital,The Third Military Medical University,Chongqing400038 ) 　　ABSTRACT　OBJECTIVE:To establish a HPLC assay for determining cassiaside A and B in the seeds of Cassia obtusifolia L.METHODS:The dried powder of the seeds of the plant was defatted with CHCl3 in a Sothles apparatus followed by extraction with MeOH.The MeOH extra was injected for analysis.A μ-Bondapak C18 column (3.9nm×300mm,10μm) was used with a mobile phase of acetonitrile-water-THF-acetic acid (20∶76.5∶3.0∶0.5).The flow rate was 1.0ml.min-1.A UV detector at 278nm was used.The peaks of cassiaside A and B were identified by co-chromatography and spectrophoto metry with their standards.RESULTS:The peas of cassiaside A and B in the chromatogram from seeds C. obtusifolia are pure.The retention time of A and B were 12.13 min and 13.27 min,respectively.The standard curves of A and B were linear in the range from 0.25 to 1.25 μg (r=0.9999) and 0.125 to 0.625 μg (r=0.9999),respectively.The average recoveries were (96.83±3.53)% for A and (97.78±2.16)% for B.CONCLUSION:The method is sensitive and specific.It is suitable for analysis of cassiaside A and B in the medicinal materials. 　　KEY　WORDS　cassiaside A and B,Cassia obtusifolia L,HPLC 　　决明子为豆科植物决明(Cassia obtusifolia L.)的干燥成熟种子［1］。有祛风散热，清肝明目，润肠通便之功效。决明子中含有多种蒽醌类成分和萘并吡喃酮类成分［2～3］。据报道［3～4］决明子中某些蒽醌苷和萘并吡喃酮苷具有对抗四氯化碳和半乳糖胺对原代培养小鼠肝细胞毒害的作用。现代药理