3、Nat Commun 2017-非编码RNA通过ceRNA介导的miRNA逃避参与cldn4调控网络.pdf

ARTICLE DOI: 10.1038/s41467-017-00304-1 OPEN Non-coding RNAs participate in the regulatory network of CLDN4 via ceRNA mediated miRNA evasion 1 1 1 1 1 1 1 Yong-xi Song , Jing-xu Sun , Jun-hua Zhao , Yu-chong Yang , Jin-xin Shi , Zhong-hua Wu , Xiao-wan Chen , Peng Gao1, Zhi-feng Miao1,2 Zhen-ning Wang1 Thousands of genes have been well demonstrated to play important roles in cancer progression. As genes do not function in isolation, they can be grouped into “networks” based on their interactions. In this study, we discover a network regulating Claudin-4 in gastric cancer. We observe that Claudin-4 is up-regulated in gastric cancer and is associated with poor prognosis. Claudin-4 reinforce proliferation, invasion, and EMT in AGS, HGC-27, and SGC-7901 cells, which could be reversed by miR-596 and miR-3620-3p. In addition, lncRNA-KRTAP5-AS1 and lncRNA-TUBB2A could act as competing endogenous RNAs to affect the function of Claudin-4. Our results suggest that non-coding RNAs play important roles in the regulatory network of Claudin-4. As such, non-coding RNAs should be considered as potential biomarkers and therapeutic targets against gastric cancer. 1 Department of Surgical Oncology and General Surgery, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang City 110001, China. 2 Division of Gastroenterology, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA. Yong-xi Song, Jing-xu Sun and Jun-hua Zhao contributed equally to this work. Correspondence and requests for materials should be addressed to Z.-n.W. (email: josieon826@) NATURE COMMUNICATIONS | 8: 289 | DOI: 10.1038/s41467-017-00304-1 | /naturecommunications 1