《进化生态学》适应性.ppt

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * But now ,many models have demonstrated that the trend of diurnal t range is on the decreasing . And from this graph showed us ,that the minimum t increased higher than maximum t., about 2-3 times. * * * * * * * * * * * * * * * * Fig.5. Schematic representation of dopamine signal transduction pathway (Fienberg et al ,Science,1998) Small RNA regulation of phase change dopamine pathway miRNA ? ? ? solitary gregarious population density Target prediction Drosophila Locust Yang ML et al, 2013, PLoS Genetics Expression pattern of miR-133 and miR-994 miR-133 Yang ML et al, 2013, PLoS Genetics Henna Target prediction of miR-133 Pale Henna CDS Yang ML et al, 2013, PLoS Genetics Expression pattern of miR-133, henna and pale miR-133 G S Yang ML et al, 2013, PLoS Genetics Expression pattern of miR-133, henna and pale miR-133 miR-133 localization in the brain Henna localization in the brain Anti- henna Control Anti- pale Control Pale localization in the brain Expression pattern of miR-133, henna and pale in the protocerebrum Whether did miR-133 interact with henna/pale？ How did miR-133 interact with henna/pale？ Question Vectors used in luciferase assay for 3UTR for mir-133 Yang ML et al, 2013, PLoS Genetics miR-133 interacts to pale and henna directely in vitro Synthetic constructor generation Yang ML et al, 2013, PLoS Genetics mRNA expressions of henna and pale in the co-transfected S2 cells Yang ML et al, 2013, PLoS Genetics Protein expressions of henna and pale in the co-transfected S2 cells Rip interaction between miR-133 and henna/pale in vivo Rip Interaction between miR-133 and henna/pale in vivo Yang ML et al, 2013, PLoS Genetics Whether did miR-133 regulate the expression of henna/pale in vivo？ Whether did miR-133 control the phase transitions of locusts？ Question Assessing the effects of miR-133 overexpression/knockdown Yang ML et al, 2013, PLoS Genetics mRNA expression levels of henna a