分子生物学课件第八章 转录后加工.pptVIP

Proteins needed in cleavage and polyadenylation of a pre-mRNA ? cleavage and polyadenylation specificity factor (CPSF)---bind to the AAUAAA signal; ? cleavage stimulation factor (CstF)--- bind to the GU region; ? cleavage factors I and II (CF I and CF II); ? poly(A) polymerase(PAP); ? PNA pol II, particularly the CTD; ? poly(A)-binding protein II (PAB II) Procedure of polyadenylation ? first phase---depend on the AAUAAA signal, slow addition of at least 10 As to the pre -mRNA. (PAP and CPSF) ? second phase---independ on the AAUAAA signal, but depend on the oligo(A) added in the first pha