组织工程及再生医学基本概念及应用范围.pptVIP

Lancet. 2006 Apr 15;367(9518):1241-6. 组织工程膀胱临床应用 Lancet.?2014 Jan 18;383(9913):238-44. 组织工程气管临床应用 小结 组织工程、干细胞和生物材料是再生医学的重要技术手段 再生医学已经从理论研究迈向临床应用 发育生物学、转基因动物及基因修饰等技术将为组织/器官再生增添新的动力 基础与机制的研究是未来突破的保障 Thank You ! * Figure 1. Secretory cells proliferate after basal cell ablation a, Schematic representation of the ablation of CK5-expressing basal cells of the trachea. Secretory, ciliated and basal stem cells are shown in pink, blue and grey colors respectively. b, Schematic of the timeline of i-dox or i-PBS administration and tissue harvest. c, Immunostaining for basal (p63 (green) and CK5 (cyan)) and secretory cells (SCGB1A1 (green)) in combination with Ki67 (red) on either i-PBS (upper panels) or i-Dox (lower panels) treated mice (n=6). White arrows, Ki67+ cells. d, Quantification of the percentage of p63+ and SCGB1A1+ cells per total DAPI+ cells in i-PBS or i-Dox n=3. e, Percentage of p63+Ki67+ and SCGB1A1+Ki67+ cells relative to total Ki67+ cells in i-PBS and i-Dox (n=3) treated CK5-DTA mice. i-Dox, inhaled doxycycline; i-PBS, inhaled PBS. Nuclei, * Figure 2. Luminal secretory cells dedifferentiate into basal stem cells after stem cell ablation a, Schematic representation of tamoxifen (Tam) and i-dox administration to Scgb1a1-YFP/ CK5-DTA mice followed by tissue harvest (H). b, Immunostaining for CK5 (red) (left panels) and NGFR (red) (right panels) in combination with YFP (green) in i-PBS (upper panels) or i-Dox (lower panels) treated mice (n=3). White arrows indicate double positive cells. c, Flowcytometric analysis of dedifferentiated cells. EpCAM+GSIβ4+CD24- cells were analyzed for expression of SSEA1 and YFP in either i-Dox and i-PBS treated mice. i-Dox, inhaled doxycycline; i-PBS, inhaled PBS. Nuclei - DAPI (blue). n=3 (at least 2 mice each per condition). Scale bar, 20μm. * * Figure 1 | A combination of three transcription factors induces insulin1 cells in adult mouse pancreas in vivo. a, Schematic diagram of the experimental strategy. Adenoviruses encoding