“金青芪颗粒”的研制及其药效学评价.pdfVIP

摘 要 肉鸡传染性腺胃炎在当今肉鸡养殖行业中属于高发和多发疾病，由于该病的发病 原因和发病机制尚不明确，给临床防治带来了极大的困难，也给肉鸡养殖带来严重的 经济损失。本试验通过中兽医群体辨证定义该病为肉鸡中焦湿热证，运用扶正祛邪的 治则建立“金青芪颗粒” 的组方，通过临床药效学试验对其疗效及作用机制进行研究。 主要试验内容及结果如下： 1. 黄芪和茯苓的提取优化：根据料液比、提取时间、提取次数设计单因素试验，后 根据单因素试验结果，设计正交试验。结果：黄芪最佳提取工艺为 15 倍量水回流提 取两次，每次30min ；茯苓最佳提取工艺为25 倍水回流提取2 次，每次2h 。 2. 颗粒剂工艺研究：根据成型效果考察不同辅料制粒结果，筛选出辅料，继而在根 据《中华人民共和国兽药典》2015 版中对颗粒剂的要求与生产实际成本，筛选最适 药物与辅料的比例。结果：当药物选择糊精为辅料，药物与辅料的比例为 1:1.5 时， 为最优成型制粒工艺，确定其为“金青芪颗粒” 的配方。 3. “金青芪颗粒”质量标准的制定：根据《兽用中药、天然药物质量标准分析方法验证 指导原则》的要求，对“金青芪颗粒”进行质量标准制定。结果：建立了简便、成熟的 “金青芪颗粒” 的质量标准。 4. “金青芪颗粒”的药效学评价：选取临床患有传染性腺胃炎的5 日龄817 肉鸡，分为 自然发病组、“金青芪颗粒”颗粒高、中、低三个用药治疗组，并以5 日龄健康817 肉 鸡做对照。结果：“金青芪颗粒”对传染性腺胃炎有较好的治疗作用，在治疗传染性腺 胃炎的过程中能够起到抗氧化、抗炎以及提高体液免疫的作用。 关键词：肉鸡传染性腺胃炎；金青芪颗粒；制备；质量标准；药效学 I Abstract Today, transmissible Proventriculitis is high-incidence and frequently-occurring diseases in broiler farming industry, which brings great difficulties to clinical prevention and treatment, as well as serious economic loss to broiler farming due to the unclear causes and pathogenesis of these diseases. By using the method of group differential diagnosis of Chinese traditional veterinarians, we defined such diseases as the Syndrome of Dampness-heat in Middle-Jiao in broilers, and established “Jinqingqi Granule” through the treatment of strengthening the body and expelling pathogenic factors. This subject mainly observes the pharmacological effects of “Jinqingqi Granule”, and research on its effective mechanism. The main study contents and results are as follows: 1. Extraction optimization of Astragalus and Poria cocos: The single factor test was designed as the ratio of solvent to material (S/M), extract time and number of extractions, and then orthogonal test was designed acco