拳参正丁醇提取物对异丙肾上腺素致大鼠心肌肥厚的保护作用.docxVIP

拳参正丁醇提取物对异丙肾上腺素 致大鼠心肌肥厚的保护作用 【摘要】 目的观察拳参正丁醇提 取物对异丙肾上腺素所致大鼠心肌肥厚的 保护作用及探索其相关作用机制。方法采用 ISO 1 mg - kg-1 ? d-1 ,背部皮下注射，连 续10 d,建立大鼠心肌肥厚模型。造模第 2 天开始给大鼠灌胃不同浓度的拳参正丁醇 提取物或N连续14 d ,末次给药后禁食 12 h,称重，麻醉后断头取血，取心脏称重 后计算全心重量指数及左心重量参数； 采用 试剂盒测定心肌组织 NOS舌性及MDA^量。 结果ISO模型组大鼠心脏重量参数及左心室 重量指数明显提高，左心室 NOS含量降低， MDA含量升高；与模型组相比，拳参正丁醇 提取物治疗组左心室重量指数下降， 心脏重 量指数有下降趋势，心肌组织NOS舌性显着 升高及MDA含量降低。结论拳参正丁醇提取 物对异丙肾上腺素所致大鼠心肌肥厚具有 保护作用，其作用机制与清除 OFF^提高NO 水平有关。 【关键词】 拳参正丁醇提取物； 异丙肾上 腺素；心肌肥厚 Abstract : ObjectiveTo observe the protective effect of Polygonum bistorla L. n-Butyl Alcohol Extract (PBNA) on myocardial hypertrophy caused by isopropylarterenol (Iso) in rats and explore its possible mechanism. MethodsThe myocardial hypertrophy model was established through hypodermic injection for 10 consecutive days at back skin with Iso, 1 mg/.Once the model was formed, the rats were administrated with various concentrations of PBNA or NS in intragastric way from the second day for 14 days. 12 h after the last administration,blood and heart were taken under anesthesia, the indexes of whole heart and left ventricle were calculated and NOS activity and MDA level of myocardial tissue were determined with kits. ResultsThere was obvious difference between Iso group and Ns group. Indexes of whole heart and left ventrical in Iso group were much higher than those in control one, NOS activity was decreased while MDA concentration was increased in left ventricle. However, both indexes of whole heart and left ventricle were decreased in BAEBgroup, furthermore, NOS activity was strikingly enhanced and MDA concentration was reduced in PBNAgroup. ConclusionBAEB has a protective effect on myocardial hypertrophy caused by Iso, and its mechanism may be related with clearance of OFR and NO concentration promotion. Key words : Polygonum bistorla L. n-Butyl alcohol extact; Isopropylarterenol; Myocardial hypertrophy 中药拳参具有清热、镇惊、利湿等功效， 主要成分为没食子酸、丁二酸、榔皮素、W 皮素-5-O- 3 -D-葡萄糖昔、原儿茶酸等［1, 2］。有实验表明，其正丁醇提取物 （Polygonum bistorla L. n-Butyl Alcohol Ext