有关蛋白质的实验技术及实验心得集锦.docVIP

有关蛋白质的实验技术及实验心得集锦 一、证明蛋白与蛋白的直接作用 用immunoprecipitation只能证明两蛋白之间有作用,但不能证明直接还是间接吧. Comment 1: Yeast 2-hybrid Or FRET Comment 2: yeast two-hybrid cant be used to definitely say protein A and protein B are directly interacted. To say two proteins are directly interacted, there are a lot of things you should take care. (1) yeast 2-hybrid was used to screen candit protein that may interact with yoru protein. A of of artificial positive exist. because : both in yeast 2-hybrid and mammalian 2-hybrid, you have overexpressed something to such an abundant extent. some RNA binding proteins bind bnd R