甘蓝型油菜菌核病抗性基因和调控途径鉴定.pdfVIP

Identification of resistant genes and pathways involved in resistance for Sclerotinia stem rot in Brassica napus CONTENTS ABSTRACT i 摘要iii List of Abbreviations v 1. REVIEW OF LITERATURE 1 1.1 General Introduction 1 1.2 Factors hindering the potential production of B. napus 2 1.3 Diseases of B.napus 3 1.4 Sclerotinia sclerotiorum impact on B. napus 3 1.5 Control of S. sclerotiorum in B. napus 4 1.5.1 Chemical control 5 1.5.2 Biological control 6 1.5.3 Cultural control 7 1.5.4 Genetic control 7 1.6 Impact of flowering time on SSR resistance in B. napus 15 1.7 Aims and objectives 15 2. MATERIALS AND METHODS 17 2.1 Plant material 17 2.2 Phenotyping measurements and statistical analysis 17 2.3 SNP array genotyping 18 2.4 Linkage mapping and QTL map construction 18 2.5 Sampling for RNA-seq analysis and metabolites profiling 18 2.6 RNA quantification and qualification, cDNA library construction and sequencing 19 2.7 Quality control and reads mapping to the reference genome 19 2.8 Differential gene expression quantification 20 2.9 qRT-PCR analysis for the validation of RNA-seq data 20 2.10 MapMan analysis for biotic stress genes 21 2.11 GO and KEGG enrichment analysis of differentially expressed genes 21 2.12 Metabolites Extraction 21 2.13 UHPLC-MS/MS Analysis 22 2.14 Database search for metabolites 22 2.15 Data Analysis of metabolites 22 I Huazhong Agricultural University (2020) Doctoral Dissertation 3. RESULTS24 3.1 Phenotyping SSR resistance in the DH population 24 3.1.1 Correlation among stem width and lesion length 26 3.1.2 Correlation between flowering time and disease resistance 26 3.2 QTL mapping 27 3.3