利用CRISPR_CAS9系统鉴定油菜BnaEOD3_CYP78A6基因的功能.pdfVIP

Functionalcharacterization ofBnaEOD3/CYP78A6inBrassicanapusbyusing CRISPR/Cas9 system Contents 摘要i ABSTRACTiii List ofAbbreviationsV 1Introduction1 1.1Theintroduction ofrapeseed1 1.1.1Production ofrapeseed1 1.1.2 Origination ofB.napus3 1.2 Theimportanceandgeneticcontrolofseed sizeinplants5 1.2.1Development of seed sizeinplants6 1.3Controlofseed sizebymaternaltissues7 1.3.1Controlofseed sizebytheubiquitin-proteasomepathway7 1.3.2 Controlofseed sizebytheDA1pathway8 1.3.3Theanaphase-promotingcomplexregulator SAMBA limitsseedgrowth15 1.3.4 Controlofseed/grain sizeby G-protein signaling15 1.4 Controlofseed/grain sizebytranscriptionalregulators19 1.4.1TheNGATHA-LIKE-KLUHpathway19 1.4.2 ThePEAPOD-KIX regulatorymodule19 1.4.3APETALA2-typetranscription factors20 1.5Controlofseed sizeby zygotictissues20 1.5.1TheHAIKUpathway controlsseed sizebyregulatingendosperm development 21 HAIKU1,HAIKU2,MINISEED3,and SHORTHYPOCOTYLUNDER BLUE1act inthesamegeneticpathway tocontrol seed sizeby influencing endosperm development21 Abscisicacid andbrassinosteroidcontrols sizebyregulatingtheHAIKU pathway22 Cytokinin actsdownstream oftheHAIKUpathway tocontrol seed size...22 1.6SHB1positivelyregulate seed sizeinplants23 1.7RoleofAGB1in seed sizeandplant growth development………………………….24 1.8EDO3isapositiveregulator topromote seed sizeinplants25 1.9Briefhistory ofgenomeediting 26 I HuazhongAgriculturalUniversity (2020)DoctoralDissertation 1.9.1Overview androleofgenomeediting27 1.10Comprehensive study ofCRISPR/Cassystem28 1.11Inducedmutationthrough CRISPR/Cas9 system for crop improvement31 1.12Aimsofthe study34 1.13Objectiveofthe s