长春花、柑橘离体再生和农杆菌介导的遗传转化研究.pdfVIP

Huazhong Agricultural University (2020) Master’s Thesis Tables of contents List of figures...…….. 6 List of tables…….… 8 Abstract…………… 9 摘要………………… 11 List of abbreviations 13 Chapter 1 Review of Literature .. ………………………………………………………15 1.1 Overview of in vitro culture in C. roseus 15 1.1.1 Applications of in vitro culture in C. roseus 17 1.1.2 In vitro culture of cell suspension from C. roseus 18 1.1.3 Plant growth regulators 19 1.1.4 An efficient genetic transformation of C. roseus 19 1.1.5 Effective regeneration/plant transformation techniques of C. roseus 20 1.2 Genetic improvement of citrus 23 1.2.1 Challenges for the citrus industry 26 1.2.2 Citrus breeding 27 1.2.3 Citrus conventional breeding 28 1.2.4 Citrus mutation breeding 28 1.2.5 Citrus transformation technologies 29 1.2.6 Genetic transformation as a tool for citrus disease-resistant breeding 31 1.2.7 Prospective citrus breeding technologies 32 1.2.8 Primary citrus diseases 34 1.2.9 Huanglongbing disease of citrus 35 1.2.10 Red fluorescent protein used genetic transformation of C. roseus 38 1.2.11 Aim and scope of the study 39 Chapter 2 In vitro regeneration and genetic transformation of C. roseus .. …………40 2.1 Materials and methods 40 2.1.1 Plants materials 40 2.1.2 Surface sterilization method 40 2.1.3 Preparation of growth media 40 Murashige and Skoog medium 40 ½ MS medium 41 2.1.4 Callus Induction 41