傅里叶变换红外光谱诊断地中海贫血症3.docxVIP

百度文库? 百度文库?让每个人平等地捉升口我 PAGE PAGE # 傅里叶变换红外光谱诊断地中海贫血症 【摘要】 为建立简单快速的地中海贫血诊断方法，本研究探讨了以傅里叶变换红外光 谱结合水平衰减全反射(FTIR HATR)技术在地中海贫血诊断中的制样方法及光 谱的数据处理方法。在制样预处理中，通过对样品进行稀释并干燥成膜消除水分 子对光谱吸收干扰，保持ATR光谱中各波长对样品的穿透深度一致。结果表明， 当1632 cm- 1吸收度小于1?5时(即透射率T小于4%时)，各波峰強度与血红蛋 白浓度呈良好的线性关系(r0. 995)及实验重复性(RSD〈4%)。在数据处理上， 改进的相对强度方法用于800?1780 cm- 1和2480?3600 cm- 1区间的分析。 通过与常规的傅里叶去卷积谱及差谱方法相比，本方法可消除样品浓度所带来的 影响因素，灵敬地揭示群体数据中组分与结构在不同组间的显著差异，如1638 cm -1处重叠的蛋口二级结构峰，1172 cm- 1 . 1440 cm- 1表征脂类物质的吸收 峰，1064 cm— 1表征磷酸化合物峰位及表征SH的2553 cm— 1附近的吸收峰在 正常组与地中海贫血组间存在显著差异。从而避免了儿个峰位的相对强度所反映 的信息不足及选择参比峰的困扰，对揭示整体的差异变化规律有着重要的作用。 【关键词】 傅里叶变换红外光谱，水平衰减全反射，地中海贫血，相对强度 Identification of Thalassemia by Fourier Transform Infrared Spectroscopy and Spectral Data Processing PENG Li Xin, WANG Gui Wen , YAO Hui Lu, HUANG Shu Shi, WANG Yi B ing, TAO Zhan Hua, LI Yong Q ing 1 (Biophysics Laboratory, Guangxi Academy of Sciences, Nanning □30003) 2(Department of Physics, East Carolina University, Greenvile, NC 27858 4353, USA) Abstract To set up a rapid, simple diagnosis of thalassemia, Fourier transform infrared spectroscopy (FTIR) associated with horizontal attenuated total reflectance (HATR) was firstly used to diagnosis B thalassemia patients. Here, the sample preprocessing and data processing were discussed in detai 1. Properly diluted samples were filmed on ATR crystal surface and then all evanescent waves can penetrate the sample completely. The IR spectra indicated excellent linearity (r0. 995) and reproducibility (RSD4%) under 1. 5 absorbance unit at 1652 cm— 1 ? Modified intensity ratios met hod was used in 800 — 1780 cm— 1 and 2480 — 3600 cm— 1 regions respectively. It demonstrated protein secondary structure change near 1638 cm— 1 and others significant differences such as bands at 1172 cm— 1 and 1440 cm— 1 that distribute to lipid, band at 1064 cm— 1 arising from phosphate substance and 2553 cm— 1 that originating from SH stretching vibration. This method not only avoids the problems of large number of free parameters comp