胎儿胸腺发育中弥散神经内分泌细胞的表达及其意义.docVIP

胎儿胸腺发育中弥散神经内分泌细胞的表达及其意义 摘 要 目的： 检测胎儿胸腺发育屮弥散神经内分泌细胞(diffuse neuroendocrine cells, DNES细胞)分布和表达特征，探讨ONES细胞对胎儿胸腺发育的生物学作用及 其意义，为神经内分泌免疫网络理论提供形态学资料。 方法： 15?40周胎儿胸腺共27例，分别进行HE染色、免疫组化染色和免疫荧光双重 染色。抗嗜珞素A (Chromogranin A, CgA)、抗神经元特异性烯醇化酶(Neuron specidic-enolase, NSE)标记 DNES 细胞，抗牛长抑索(Somatostatin, SS)和抗血管活 性肠肽(Vasoactive intestnal peptide, VIP)检测DNES细胞的内含物。通过图像分析系 统检测各胎龄组DNES细胞面数密度。 结果： 免疫组化染色 CgA和NSE阳性细胞在15?38周胎儿胸腺均表达，27?28周前随胎龄增加表 达增多，之后减少，39周及40周未见表达。两者表达特征相似，它们主要散在分 布于髓质胸腺细胞之间，细胞呈圆或椭圆形；分布于胸腺小体周边，细胞呈圆、椭 圆或三角形。SS和VIP阳性细胞在15周胸腺即表达并持续至40周，在17?18周 表达较旺盛，分布于胸腺髓质及皮髓交界处，呈圆或椭圆形。 免疫荧光双标染色 胎儿胸腺发育屮CgA、NSE、SS和VTP阳性细胞均有表达，分布及形态特征同 免疫组化相似。部分CgA和NSE, CgA和SS, CgA和VIP阳性细胞分别重叠。 结论： 1?胎儿胸腺发育屮DNES细胞发育活跃，尤其在胚胎发育屮期，表明其可能对胸腺发 育和功能建立具有重要影响。 2. DXES细胞在胸腺髓质的优势分布，提示其可能参与胸腺细胞发育、分化、成熟及 凋亡等过程。 关键词： 胎儿、胸腺、弥散神经内分泌系统、嗜锯素A、神经元特异性烯醇化酶、生长 抑素、血管活性肠肽 Expression and Significance of the Diffuse Neuroendocrine Cells in the Development of Fetal Thymus ABSTRACT Objective： To observe the expression and the distribution of the diffuse neuroendocrine cells in fetal thymus and to discuss its biological significance in fetal thymus. Methods； 27 specimens of fetal thymus (from 15lh week to 40lh week of gestation) were obtained and stained by immunohitochemican staining and immunofluorescent double staining. Neuroendocrine cells were identified by immunohistochemical staining of chromogranin A (CgA) and neuron specific enolase (NSE). Somatostatin (SS) and vasoactive intestinal peptide(VIP) were used to identify the inclusions of neuroendocrine cells. The density of neuroendocrine cells was assessed by microimag e analyzing system. Results： The positive CgA and NSE cells were expressed in fetal thymus from 15th week to 38th week. The number of them increased with the gestational weeks until the 28,hweek and then decreased. And both of them were not detected in fetal thymus from 39th week to 40,h wcck.Thc immunoreactive CgA and NSE cells were highly located at the medullary of fetal thymus displaying a simple round,oval and