不同光周期对航空诱变凤仙花幼苗生长的影响.docVIP

不同光周期对航空诱变凤仙花幼苗生长的影响 摘 要：为探究光周期对不同诱变世代是否有作用以及如何作用，本研究通过人工控制光照时间, 观察并测定了不同光周期处理（L15D9、L12D12、L9D15）对普通凤仙花幼苗、航空诱变凤仙花SP5代和SP8代幼苗形态和生理指标的影响。结果表明，延长光照时间可提高三种凤仙花幼苗叶面积、根冠比（R/T）及叶绿素含量，而可溶性蛋白含量随光照的延长而先升高后降低，其中光照12h时，凤仙花可溶性蛋白合成相对敏感。航空诱变凤仙花SP8各项指标显著高于对普通凤仙花 (p0.05)，同时又显著低于航空诱变凤仙花SP5(p0.05)，这说明光周期对不同诱变世代有作用，光照时间的延长可以适当弥补航天诱变凤仙花第八代的性状减弱。 关键词：凤仙花；光周期；航空诱变；幼苗 The effect of different photoperiod on the aerospace mutation Impations balsamina seedling Abstract:In this study, to explore photoperiod whether there is a role for the different mutation generations and how to work，The effect of different photoperiod（L15D9、L12D12、L9D15）on morphological and physiological indicators of Impations balsamina that SP5 generation space mutation and SP8-generation space mutation and nature plants were observed and determined by artificial controlling illumination time. The results showed that:Leaf area,R/T,chlorophyll content were increased by prolong the illumination time,while the soluble protein content in the early phase of illumination time and decreased subsequently,and the relatively sensitive to photoperiod of the Impations balsamina’s soluble protein synthesis is 12 hours. The indexes of SP8 generation Impations balsamina seedling of space mutation significantly higher than those of nature Impations balsamina seedling(p0.05),and significantly lower than SP5 generation Impations balsamina seedling of space mutation(p0.05).This suggests that the photoperiod has a role for the different mutation generations,and Prolonged illumination can compensate for the weakening of traits in mutagenesis groups appropriately. Key words:Impations balsamina;Photoperiod;Space mutation;seedling 目录 1前言…………………………………………………………………………………1 2材料与方法…………………………………………………………………………1 2.1实验材料…………………………………………………………………………1 2.1.1实验样品………………………………………………………………………1 2.1.2主要实验仪器…………………………………………………………………2 2.1.3实验试剂………………………………………………………………………2 2.2幼苗的培养和光周期处理………………………………………………………2 2.3形态指标的测定…………………………………………………………………2 2.4生理指标的测定…………………………