分子生物学资料：Chapter6_Questions(1).pptVIP

? 2014 Pearson Education, Inc. MOLECULAR BIOLOGY OF THE GENE ? 2014 Pearson Education, Inc. Seventh Edition Watson ? Baker ? Bell ? Gann ? Levine ? Losick Questions by Bryan Jennings and James Lodolce Chapter 6 课后作业 2014-11-17 1. Under which set of conditions does the lac operon in E. coli produce high levels of lacZ, lacY, and lacA transcripts? in the presence of lactose and absence of glucose in the presence of both lactose and glucose in the absence of both lactose and glucose in the absence of lactose and presence of glucose 2. In prokaryotes, when can basal level (constitutive) expression of a gene occur? only when an activator binds when an activator and repressor bind in the absence of activator and repressor binding only if the expression of the gene is regulated by a repressor 3. You create an E. coli mutant that has a two bp deletion between the –10 and –35 elements of the merT promoter. In the absence of mercury, which of the following outcomes do you expect? expression of merT, but only in the presence of the activator MerR no expression of merT constitutive expression of merT expression of merT, but only in the presence of the activator MerR 4. Which one of the following statements is false regarding the role of ? repressor in the regulation of phage transcription? ? repressor bound to DNA excludes RNA polymerase. ? repressor cooperatively binds DNA. ? repressor lacks an activation domain. ? repressor recruits RNA polymerase to the promoter. 5. Which of the following statements is true regarding cooperative binding? It decreases the concentration of protein required to fill adjacent protein binding sites on DNA. It increases the concentration of protein required to fill adjacent protein binding sites on DNA. It decreases the affinity of a protein for its correct binding site on DNA. It is observed only in DNA-binding proteins. 6. AraC and CAP both regulate transcription of the araBAD operon. Which statement best describes the role of these