d酶固定化蛋白质工程.pptxVIP

d酶固定化蛋白质工程会计学Understanding enzyme immobilisation Enzymes are versatile catalysts in the laboratory and on an industrial scale. To broaden their applicability in the laboratory and to ensure their (re)use in manufacturing the stability of enzymes can often require improvement. Immobilisation can address the issue of enzymatic instability. Immobilisation can also help to enable the employment of enzymes in different solvents, at extremes of pH and temperature and exceptionally high substrate concentrations. At the same time substrate-specificity, enantioselectivity and reactivity can be modified. However, most often the molecular and physical-chemical bases of these phenomena have not been elucidated yet. Chem Soc Rev. 2009 Feb;38(2):453-68. /sites/entrez?Db=pubmedCmd=SearchTerm=%22Hanefeld%20U%22%5bAuthor%5ditool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DiscoveryPanel.Pubmed_RVAbstractPlusHanefeld U, /sites/entrez?Db=pubmedCmd=SearchTerm=%22Gardossi%20L%22%5bAuthor%5ditool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DiscoveryPanel.Pubmed_RVAbstractPlusGardossi L, /sites/entrez?Db=pubmedCmd=SearchTerm=%22Magner%20E%22%5bAuthor%5ditool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DiscoveryPanel.Pubmed_RVAbstractPlusMagner E.Recent advances in immobilized enzymatic reactors and their applications in proteome analysisImmobilized enzymatic reactors recently have drawn much attention because of the striking advantages, such as high substrate turnover rate and ease in coupling with the separation and detection systems. Carrier materials, which have great effects on the development of the immobilized enzymatic reactors, have always being the focus of study. In this paper, the contributions, mainly in the last 5 years, on the enzymatic reactors and their applications in proteome study are reviewed, with some newly developed inorganic and organic carriers for enzyme immobilization described in details. Moreover, the hyphenation of immobili