分光光度法测定自来水中镁.docVIP

分光光度法测定自来水中镁 分光光度法测定自来水中镁 分光光度法测定自来水中镁 分光光度法测定自来水中的镁 摘 要 本实验是应用分光光度法测定自来水中镁含量。在 pH值为 10的氨水 -氯化铵碱 性缓冲液中，以 MTB （甲基百里香酚蓝）作为显色剂，镁与 MTB 生成稳固的蓝紫色 络合物，其吸光度与镁含量在必定浓度范围内成正比。在室温条件下，用 721B型分 光光度计，在 602nm波优点、 4mLpH值为 10的氨水 -氯化铵碱性缓冲液、 3mL的 MTB(0.3784 μg/mL) 溶液、显色时间 8分钟测定吸光度， 络合物的吸光度达到最大值 而且相对稳固。 实验结果表示： 镁浓度在 1.00~5.00ug mL· -1呈优秀的线性关系获得标 准曲线的线性回归方程为 （x：镁含量， y：吸光度），有关系数 为0．9995，摩尔吸光系数为 1.79 ×104L/mol ·cm，实验对 16种扰乱离子进行了观察， 发现标准偏差在 5%范围外的有钙、锌 2种离子对镁测定结果存在较大扰乱，加入 EGTA（乙二醇二乙醚二胺四乙酸）和六次甲基四胺为遮蔽剂，能够分别除去钙离 子和锌离子的扰乱。最后试验测得自来水中镁离子含量为 28.25g/L,均匀回收为 99.63%，镁离子测定比较正确。 重点词 ：分光光度法，镁， MTB ，自来水 ,EGTA ，六次甲基四胺 I SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF WATER MAGNESIUM Abstract a method for determining in magnesium content in water with spectrophotometry is proposed in this paper. MTB is selected as the chromomeric reagent. Magnesium and MTB generate amaranth complex. In the medium(pH=10) of Ammonium Hydroxide –ammonium chloride acetate, magnesium combined with MTB to form a stable quaternion–mixed micelles system with the existence of polyethylene glycol The absorbency was direct proportion to the content of magnesium in a certain range of concentration. It has been determined the wavelength of maximum absorption is 602nm. At room temperature, with 721 type spectrophotometer to measure spectrophotometry, through the experiment determination of the stability and reproducibility worse after the -4 test, 5 10×μ gmL-1 the MTB in solution 3 mL sensitive, and measure the highest when at room temperature, show color time after 8 minutes, in the pH value of 10 Ammonium Hydroxide –ammonium chloride alkaline solution in the complex, the absorbency and reach a maximum relatively stable. The experimental results show that: magnesium concentration in 1.00 ~5.00ug/mL is good linear relationship between the get standard curve of the linear regression equation is Y=0.0632X+0.1626, correlation coefficient is 0.9915, the molar absorption coefficient is 7.1 × 104 L / mol cm,· molar absorption coefficient is 7.