蒲芍前列腺炎胶囊乙醇提取最佳工艺研究.docVIP

蒲芍前列腺炎胶囊乙醇提取最佳工艺研究 ［摘要］目的确定蒲芍前列腺炎胶囊的乙醇提取最佳工艺。方法 采用正交试验法，以丹参酮IIA和芍药廿提得量为指标，対乙醇浓度、乙 醇用量、提取时间及提取次数4个影响因素进行考察，优选提取工艺。结 果以80%乙醇加热冋流提取3次，每次1.0 h,每次加醇量分别为6倍为 提取条件时，所得丹参酮IIA和芍药昔最高，此为蒲芍前列腺炎胶囊的乙 醇提取最佳工艺。结论 优选出的最佳工艺条件经验证实验表明结果稳 定，指标性成分含量高，可以用于投入生产。 ［关键词］蒲芍前列腺炎胶囊；丹参酮IIA；芍药昔；提取工艺；正交 设计 ［中图分类号］TQ461：文献标识码］A［文章编号］1673-7210 (2014) 06 (b) -0100-04 Study on elhanol extraction technique of Pushaoqianliexianyan Capsules DONG Zhengqil ZHAO IIaitingl,2 WANG Yunzhel,2 CUI Jiayinl,2 ZHONG Chonglin3 1. Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Beijing 100193, China; 2.Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China; 3.Academy of Traditional Chinese Medicine and Chinese Materia Medics of Jilin Province, Changchun 130021, China [Abstract] Objective To optimize the conditions of the extraction technique for 卩ushaoqianliexi且nyan Capsules? Methods The ethanol extraction technology was studied by using orthogonal experiment, with content of Tanshinone II A and Paeoniflorin as research index? Four factors were investigated including the concentration of ethanol, the volume of ethanol, the extraction time and the number of extracting times? ResuIts The optimum condition of ethanol process for Pushaoqianliexianyan Capsules was that the crude herb powder was extracted by 3 times with 6-fold 80% ethanol for 1 hour? Conclusion The optimized process can be used for enlarged production with good repeatability and stable quality in samples? [Key words] 卩ushaoqianliexianyan Capsules; Tanshinone II A; Paeoniflorin; Extraction technology; Orthogonal design 慢性前列腺炎(chronic prostatitis, CP)是中青年男性的常见病、 多发病，该病症状复杂，病程较长，且容易复发,严重影响患者的身心健 康[1-2] o蒲芍前列腺炎胶囊系在研的新药，是由土茯苓、蒲公英、牛膝、 丹参、赤芍、红花等十味中药组成，具有清热利湿，活血通淋之功效，用 于慢性前列腺炎(属于湿热下注证者)[3-4] o该制剂是治疗前列腺疾病 的师传经验秘方，经30多年临床实践，吸收现代医学新成果，不断总结 与改进，形成被临床许多病例验证取得满意疗效的复方制剂。 在蒲芍前列腺炎胶囊中，丹参、赤芍和红花需要乙醇提取脂溶性有效 成分，且丹参及赤芍的用药量在本方中最大，是该药处方中的主药。为此 需要对乙醇提取技术条件进行优选，以提高提取效率，最大程度获取丹参 及赤芍的有效成分，发挥蒲芍前列腺炎胶囊的药效。在现有的研究中 [5-6],通常只对丹参或赤芍的乙醇提取工艺