葛根素对去卵巢大鼠机体骨代谢影响的观察.docVIP

葛根素对去卵巢大鼠机体骨代谢影响的观察 作者：黄彤金邦荃孙桂菊杜先锋宛晓春 【摘要】目的观察葛根素对去卵巢大鼠机体骨代谢的影响，探讨 其对雌激素缺乏引起的骨质疏松症的治疗作用。方法3月龄清洁级 SD大鼠60只，背驮式切除双侧卵巢后每日灌胃葛根素5 mg/kg(P 5组),10 mg/kg(P 10组)和20 mg/kg(P 20组)，并设假手术组 (Sham),模型组(OVX)和己烯雌酚阳性对照组(E) o 3个月后处死动物, 测定大鼠胫骨干重、灰分重量和矿物质含量，胫骨Ca、P含量以及血 清相关骨代谢指标。结果与OVX组相比，葛根素各组的胫骨矿物质 含量(mg/g)均有增加(574 土 17, 590 ±22 和 597 ±18),其中 P 20 组差异显著(Plt;0. 05);葛根素各组的胫骨Ca含量(mg/g)高于OVX 组(132± 10, 222 ± 7, 228 ±8),其中 P 10,P 20 两组差异显著 (Plt; 0. 05,Plt; 0. 01),说明服用葛根素后大鼠骨量得到增加；同 时，葛根素各组的碱性磷酸酶(U/L)与OVX组有所降低(101±26, 90 ± 20 , 71 ± 15)，其中P 10, P 20两组差异显著 (Plt; 0. 05,Plt; 0. 01),说明去卵巢大鼠骨的高转换状态得到改善。 结论葛根素能抑制去卵巢大鼠骨量的丢失，对骨代谢有较好的调节 作用，对雌激素缺乏引起的骨质疏松症有一定的治疗作用。 【关键词】葛根素；去卵巢大鼠；骨代谢 [Abstract 1 Objective To investigate the effects of puerarin on the bone metabolism in ovariectomized rats, and to explore the therapeutic effect of puerarin on osteoporosis caused by the deficiency estrogen. Methods Sixty female SD rats, three month old, were ovariectomized bi lateraliy under anesthesia.Except for the sham and OVX groups, the other animals were administrated with puerarin 5, 10， 20 mg/kg or diethylstilbestrol (100 p g/kg) respectively for 3 months? The dry weight and ash weight of tibia, tibial BMC, the contents of inorganic elements in tibia were detected as wel 1 as biochemical indexes of bone metaboli sm.Results Compared wi th that of OVX group, the tibial BMC in P 20 group〔 (597 土 18) mg/gJ was increased obviously(Plt; 0.05), the contents of Ca in tibia in P 10〔(222 ± 7) mg/g〕and P 20〔(228 ±8)mg/g〕 groups were greater than those in OVX group (PIt; 0. 05, Plt; 0.01)， which meaned puerarin cou 1 d enchance the bone mass? At the same time, the ALP activity in serum in P 10 C (90 ±20) U/LJ andP 20 C (71 ±15) U/LJ groups were decreased significantly comparing to those in OVX group (Pl t; 0. 05, Plt; 0. 01). Puerarin could inhibit the high transi t ion situation of bone metabolism induced by estrogendeficiency. Conclusions Puerarin can inhibit the loss of bone and has adjust ive effects on the bone metabol