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- 约4.93千字
- 约 27页
- 2021-11-04 发布于浙江
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第一页 Plant morphology A perennial herb, main root stout. 多年生草本,主根粗壮。 Stem erect and branched at the upper part. 茎直立,上部分枝。 Leaves odd-pinnately compound, alternate. 奇数羽状复叶,互生。 Flowers in axillary racemes, calyx campanulate. 花为总状花序,腋生,花萼钟形。 第二页 Collection and preparation The drug is collected in spring and autumn, removed from soil, rootlet and root stock, and dried in sun. 春秋季节采收药材,去除泥土、细根、根头,晒干。 第三页 Description Externally pale brownish-yellow or pale brown, with irregular, longitudinal wrinkles and more transverse lenticel-like protrudings, cork easily exfoliated, exposing yellowish-white cortex, sometimes showing reticulated fibre bundles. 表面为浅棕黄色或浅棕色,有不规则纵皱纹和众多横长皮孔样突起,栓皮易剥落而露出黄白色皮部,有时可见网状纤维束。 Wood pale yellow with radiate striations and fissures, the centre part of old root occasionally rotten-wood-shaped.木部淡黄色,有放射状纹理和裂隙,老根中心偶呈枯朽状。 第四页 第五页 理化鉴别 Filter-filtrate 过滤-滤液 Neural aluminium oxide column 中性氧化铝rate Elute-elutate 洗脱-洗脱液 Residue 残渣 n-butanol saturated with water 水饱和正丁醇 The test solution 供试品溶液 CRS chemical reference standards 化学对照品 The reference solution 标准溶液 Thin layer chromatography 薄层色谱 Sulfuric acid 硫酸 formic acid 甲酸 Fluorescent spots 荧光斑点 第六页 取本品粉末1g,加三氯甲烷20ml,加热回流30分钟,滤过,滤液蒸干,残渣加三氯甲烷1ml使溶解作为供试品溶液。另取五味子对照药材1g,同法制成对照药材溶液。再取五味子甲素对照品,加三氯甲烷制成每1ml含1mg的溶液,作为对照品溶液。 第七页 To 1 g of the powder add 20 ml of chloroform, heat under reflux on a water bath for 0.5 hour, filter, evaporate the filtrate to dryness. Dissolve the residue in 1 ml of chloroform as the test solution. Produce a solution of Fructus Schsandrae Chinensis reference drug in the same manner as the reference drug solution. Dissolve deoxyschizandrin (五味子甲素) CRS in chloroform to produce a solution containing 1 mg per ml as the reference solution. 第八页 照薄层色谱法(附录VIB)试验,吸取上述三种溶液各2uL,分别点于同一硅胶GF254薄层板上,以石油醚-甲酸乙酯-甲酸(15:5:1)的上层溶液为展开剂,展开,取出,晾干,置紫外灯光下检视。供试品色谱中,在与对照药材和对照品色谱相应的位置上,显相同颜色的斑点。 第九页 Carry out the method for thin layer chromatography (Appendix VI B), using silica gel GF254 as the co
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