复方丹参注射液对受损伤的血管内皮细胞形态结构的影响.docVIP

复方丹参注射液对受损伤的血管内皮细胞形态结构的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：复方丹参注射液对受损伤的血管内皮细胞形态结构的影响 1 1 材料与方法 2 2 结果 4 3 讨论 5 文2：杏丁注射液对血管内皮细胞HSP72表达的影响 6 1 材料与方法 7 2 结果 10 3 讨论 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 复方丹参注射液对受损伤的血管内皮细胞形态结构的影响 文1：复方丹参注射液对受损伤的血管内皮细胞形态结构的影响 Effects of the Compound Injection of Dahen on the Morphological Structure of Injured Vascular Endothelial Cells Abstract：ObjectiveTo study the protective effects of the compound injection of Dan-shen on cultured endothelial cells (EC) injured by oxidization. MethodsThe morphological characteristics of EC in primary cutured human umbilical vein cells (HUVEC) in vitro were ovserved by the inverted microscope and scanning electron microscopy(SEM). The content of MDA in the supernatant fluid of cultured EC cells was detected by was observed by SEM that ECs in the H2O2 group were wrinkle and small, cell membrane had split and aperture, the microvillus decreased or disappeared, stiff or broken, and the cell gaps were wide. However, ECs in the Dahen group was similar with the normal group. MDA content obviously increased in the H2O2 group. However, the content of normal and Dahen group decreased. (P) .ConclusionThe compound injection of Dahen has protective effects on the injured vascular endothelial cells induced by oxidization through antioxidization function, Key words：Injection, Dan-shen; Vascular endothelial cell; Scanning electron microsopy 心血管疾病是人类死亡的首要原因。在各种心脑血管疾病中，很多都是由动脉粥样硬化（atherosclerosis, AS）而引起。而许多研究表明血管内皮细胞（endothelial cell, EC）损伤是AS发生的始动环节［1］。因此保护血管内皮对防治AS具有重要意义。 本实验通过扫描电镜观察细胞的超微结构，用分光光度法检测MDA含量,以探讨丹参对受损伤的血管EC的保护作用，以及其抗AS的作用机理。 1 材料与方法 药物与试剂复方丹参注射液（含丹参1 g/ml）为上海中西药液股份有限公司产品，批号021001；M199培养基为美国GIBCO公司产品；胎牛血清为美国HYCOLONE公司产品；MDA试剂盒为北京邦定公司提供；扫描电镜为日本产品。 人脐静脉内皮细胞培养按Jaffe等［2,3］人的方法略加修改进行。在无菌条件下，取健康产妇的脐带， 灌注%Ⅰ型胶原酶（collagenase-Ⅰ）10 ～15 ml，置37℃水浴中孵育15～20 min。1 000 min离心10 min，沉淀中加入含有20％FBS的完全M199培养液5 ml，充分混合制成细胞悬液，以（~）×104/cm2密度植入培养瓶中，置37℃，5％的CO2培养箱中培养，定期换液，待细胞长成融合状态后进行鉴定