复方丹参片对糖尿病大鼠坐骨神经和红细胞山梨醇浓度的影响.docVIP

复方丹参片对糖尿病大鼠坐骨神经和红细胞山梨醇浓度的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：复方丹参片对糖尿病大鼠坐骨神经和红细胞山梨醇浓度的影响 1 1 器材与方法 2 2 结果 4 3 讨论 5 文2：糖痹胶囊对糖尿病大鼠坐骨神经超微结构的影响 6 1　材料与方法 7 2　结果 8 3　讨论 9 参考文摘引言： 10 原创性声明（模板） 12 文章致谢（模板） 12 正文 复方丹参片对糖尿病大鼠坐骨神经和红细胞山梨醇浓度的影响 文1：复方丹参片对糖尿病大鼠坐骨神经和红细胞山梨醇浓度的影响 Effects of Tablet Compound Redrooted Salvia on the Function of Sciatic Nerve and the Concentration of Sorbitol in Erythrocyte on Diabetic Rats Abstract：ObjectiveTo observe the preventive and therapeutic effects of tablet Compound red rooted Salvia on early pathological changes of peripheral neuropathy in rats and to study its action mechanism. MethodsDiabetes mellitus models in rats were induced by Streptozotocin. Tablet Compound Salvia in different dosages were administered to treat the diabetic rats one weeks after being modeled. Four week later, the changes in content of FBG, GSP, and weight, content of sorbitol were observed in sciatic nerves and iide erythrocytes as well as the conduction velocity of sciatic nerve in diabetic rats. ResultsTablet Compound Salvia has the effect of reducing the content of glycoprotein in serum and content of sorbitol in sciatic nerve and in erythrocyte and increasing the conduction velocity of sciatic nerve. ConclusionTablet Compound Salvia has preventive and therapeutic effects on early pathological changes of peripheral nerve in diabetic rats. Key words：Tab Compound redrooted Salvia; Peripheral neuropathy in diabetes; Sorbitol 糖尿病周围神经病变（diabetic peripheral neuropathy, DPN）是糖尿病（DM）常见的慢性并发症之一。高血糖引起糖基化终末产物（advanced glycosylation end products, AGEs）形成增加、多元醇代谢异常是导致糖尿病周围神经病变的重要原因之一。本实验用链脲佐菌素制成大鼠DM模型，观察复方丹参片对实验性糖尿病大鼠糖化血清蛋白、坐骨神经和红细胞山梨醇水平的影响，并探讨其作用机制。 1 器材与方法 药品与试剂复方丹参片，由宁夏启元药业有限责任公司提供，批号051029（每片含丹参酮Ⅱ mg，丹参酚酸 mg），实验前粉碎，过80目筛，溶于新鲜蒸馏水中，配成适当浓度贮存于4℃冰箱中备用；链脲佐菌素，（streptozotocin, STZ）、山梨醇为美国Sigma公司产品。 仪器美国贝克曼公司DU640紫外/可见分光光度计，南京产大白鼠恒温手术台，美国罗氏公司稳豪型全微量血糖检测仪，成都泰孟公司BL420生物机能实验系统，德国Herml 2W323H低温超速离心机，宁波产DY89Ⅰ型电动玻璃匀浆机。 动物模型建立与分组SD清洁级健康雄性大鼠（250±2