复方木鸡冲剂抑制HepG2细胞增殖及诱导分化的作用.docVIP

复方木鸡冲剂抑制HepG2细胞增殖及诱导分化的作用 目录 TOC \o 1-9 \h \z \u 目录 1 正文 2 文1：复方木鸡冲剂抑制HepG2细胞增殖及诱导分化的作用 2 1 材料 3 2 方法 4 3 结果 5 4 讨论 7 文2：RhoA在洛伐他汀抑制HepG2细胞增殖中的作用 8 1 材料与方法 9 11 主要试剂、仪器 9 12 方法 10 121 细胞培养 10 122 平板克隆形成试验 10 123 总RNA提取 11 124 基因芯片检测 11 125 RT_PCR检测 11 126 Western blot检测 12 127 统计学处理 12 2 结果 12 21 平板克隆形成试验 12 22 基因芯片检测结果 12 23 RT_PCR检测mRNA(RhoA) 12 24 Western blot检测RhoA蛋白表达 13 3 讨论 13 参考文摘引言： 14 原创性声明（模板） 15 文章致谢（模板） 16 正文 复方木鸡冲剂抑制HepG2细胞增殖及诱导分化的作用 文1：复方木鸡冲剂抑制HepG2细胞增殖及诱导分化的作用 Study on inhibition proliferation and differentiation 【Abstract】 Object To study the inhibition effect and mechanism of Muji Chongji on hepatocarcinoma cell line HepG2. Methods MTT assay was adopted to describe the proliferation of carcinoma cells. Apoptosis induced by Muji Chongji was investigated by applying light microscopy and flow cytometry (FCM), The contents of γ-glutamyl trapeptidase (γ-GT) and alkaline phosphatase (ALP) in the supernatant were determined by biochemistry techniques, α-fetoprotein (AFP) was detected by radioimmunoassay. Immunohistochemical staining was performed to determine the protein of p21WAF1. Results：Our results showed Muji Chongji reduced HepG2 cell viability obviously(p); changes of apoptosis in morphology of HepG2 treated with Muji Chongji was appeared and apoptotic cells rate increased (p); significantly decreased AFP value of HepG2 secretion and increased ALP value. Our study also showed that Muji Chongji induced up-regulation of p21WAF1 protein. Conclusion Fufang muji Chongji can inhibit the proliferation of HepG2 cells and induce apoptosis in HepG2 mechanism of anti-tumor activities of Fufang muji Chongji is that induce differentiation in HepG2 cells and there is significant correlation with cell cycle inhibitor p21WAF1. 【Key words】Muji Chongji; HepG2 cell line; apoptosis; differentiation 原发性肝癌是最常见的恶性肿瘤，死亡率很高。在 发展 中国 家发病率占全球肝癌病例80%以上而我国发生的肝癌占全世界发病率的%[1]。目前肝癌的药物 治疗 中是以化学药品为主，但化学