砂仁提取液对离体家兔主动脉条收缩性能的影响.docVIP

砂仁提取液对离体家兔主动脉条收缩性能的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：砂仁提取液对离体家兔主动脉条收缩性能的影响 1 1 材料与方法 3 2 结果 5 3 讨论 6 文2：收缩阻裂砂浆强度及收缩性能的影响研究 7 1.概述 7 2.原材料与试验方案 7 3.试验结果及分析 8 4.结语 10 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 12 正文 砂仁提取液对离体家兔主动脉条收缩性能的影响 文1：砂仁提取液对离体家兔主动脉条收缩性能的影响 Effects of Amomum Villosum Extraction on the Contraction of Rabbit Thoracic Aorta in vitro Abstract：ObjectiveTo investigate effects of Amomum villosum extraction (AVE) on contraction of rabbit thoracic aorta in isotonic contraction of the thoracic aorta strips in rabbits was recorded, and the effects of AVE on the concentrationrespoe curves of noradrenalin, high potassium was observed. The procedure of freeCa2+ kerbs and Ca2+ addition were designed to indirectly observe the effects of AVE on intracellular free Ca2+ stimulated by shifted the concentration-respoe curves of NE and KCl, and the maximum respoes decreased. In Ca2+- free medium, AVE concentration-dependently inhibited the traient induced by NE and it was tended to inhibit the longlasting contraction induced by addition of Ca2+.ConclusionAVE has significant relaxing effect on the contraction of thoracic aorta strips. The effect may be due to the inhibition of the calcium channel of aortic smooth muscle. AVE shows relatively less effect on the extracellular Ca2+dependent. than that on the intracellular Ca2+dependent contraction, and more agait effects on potential dependent Ca2+ channel than recepto operated channel. Key words：Amomum Villosum Extraction； Noradrenalin； KCl； Thoracic aorta 中药砂仁为姜科植物阳春砂 Amomum villosum Lour.，绿壳砂 Amomum villosun . xanthioides . Wu et Senjen 或海南砂 Amomum longiligulare 的干燥果实，目前《 中国 药典》收载的砂仁共有3种，具有化湿开胃，温脾止泻，理气安胎的功效［1］；同时砂仁也是我国民间常用的重要香料物质。国内外对砂仁进行了大量的研究，但砂仁化学及药理活性成分研究并不同步。目前研究多集中于挥发油的药理作用［2，3］以及砂仁对胃肠道的作用［4］，由于中药临床多以水煎煮入药，其挥发油摄入量很难达到成分研究时的控制水平。 现代 的研究资料提示，理气药大多具有一定的心血管活性，其活性成分与黄酮类或异黄酮类物质相关［5］。吴师竹［6］研究发现，砂仁水煎液对花生四烯酸、胶原与肾上腺素混合剂所诱发的小鼠急性脑缺血具有显著的保护作用，孙兰等［7］从砂仁水溶液成分中提取出黄酮醇与黄酮苷类化合物、Kitajima由70%甲醇提取