口服灵芝孢子油对小鼠的扶正及减毒作用研究.docVIP

口服灵芝孢子油对小鼠的扶正及减毒作用研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：口服灵芝孢子油对小鼠的扶正及减毒作用研究 1 1 材料 3 2 方法 3 3 结果 5 4 讨论 7 文2：根痛合剂对小鼠的急性毒性作用研究 8 1实验材料 8 2.实验动物饲养管理环境 9 3.实验方法 9 4.实验结果 9 5.结论 10 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 口服灵芝孢子油对小鼠的扶正及减毒作用研究 文1：口服灵芝孢子油对小鼠的扶正及减毒作用研究 Effect of Ganoderma lucidum Spores Oil on Reducing the Toxicity of Radiochemotherapy and Enhancing the Function of Immune System in Mice Abstract：ObjectiveTo study the effects of G. lucidum spores oil on reducing the toxicity of the radiochemotherapy and enhancing the function of immune system in mice. Methods Study the effect of G. lucidum spores oil (at the dose of ，， g/kg) on reducing the toxicity of the Cy chemotherapy and 60Co radiotherapy by detecting the number of the peripheral blood leucocyte, the number of bone marrow karyota;spleen index, thymus index of normal mice and H22-bearing mice by carbon clearance test and serum erythrocytolysin test. Results It showed that G. lucidum spores oil at high ( g/kg) and middle dose ( g/kg) could significantly relieve the decrease of the number of the peripheral blood leucocyte, the number of bone marrow karyota, spleen index and thymus index of normal mice and tumopbearing mice by Cy at a high dose and 60Co (P＜, P＜). G. lucidum spores oil at each dose could significantly increase macrophage phagocytic index; and at high and middle dose it can significantly enhance the serum erythrocytolysin level of H22-bearing mice (P＜, P＜).ConclusionG. lucidum spores oil could relieve the myelosuppression caused by Cy and 60 Co; enhance the cellular and humoral immune function of tumor H22-bearing mice. Key words：G. lucidum spores oil; Immunity; Cy 灵芝是祖国医药宝库中的珍贵药品，我国最早的药学专著《神农本草经》将其列为“上品”。基于灵芝的确切临床 治疗 作用，近年来对其化学成分及作用机理进行了大量的研究，对灵芝的生殖细胞――孢子的药用价值研究也日趋深入。以往的研究表明，灵芝孢子具有显著的防癌、抗肿瘤作用，其功效是灵芝子实体的60～70倍。本实验所用灵芝孢子油是利用超临界 CO2流体萃取技术从灵芝破壁孢子中提取出来并浓缩富集而得，其各种有效成分（如三萜类）的含量均较传统提取方法显著提高。本实验主要研究其对荷瘤小鼠的免疫调节及对常规放化疗的减毒作用，以期为灵芝孢子油的临床抗肿瘤辅助应用提供实验依据。 1 材料