人参皂苷CK对四氯化碳致大鼠慢性肝损伤的影响.docVIP

人参皂苷CK对四氯化碳致大鼠慢性肝损伤的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：人参皂苷CK对四氯化碳致大鼠慢性肝损伤的影响 1 1 材料与方法 2 3 讨论 4 文2：大果山楂黄酮提取物对四氯化碳致大鼠慢性肝损伤的保护作用 5 1 器材与方法 6 2 结果 8 3 讨论 10 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 人参皂苷CK对四氯化碳致大鼠慢性肝损伤的影响 文1：人参皂苷CK对四氯化碳致大鼠慢性肝损伤的影响 Abstract:ObjectiveTo observe the effect of Compound K on chronic hepatic injury induced by CCl4 in male Wistar rats hepatic chronic injury was induced by the subcutaneous administration of CCl4 twice a week for six weeks. Then rats were given Compound K at dose of , 1 or 3 mg/kg respectively for a further four weeks. At the end the biochemical paramete associated with hepatic injury were determined, and the histopathology of liver was with the model group, activities of alanine aminotraferase (ALT), aspartate aminotraferase (AST) were significantly reduced by Compound K at dose of mg/kg, accompanied with that the amount of superoxide dismutase (SOD) markedly increased and malondialdehyde(MDA) reduced obviously, while no significant changes in the amount of hyaluronic acid (HA) and procollagen III (PCIII) or histopathology were K at low dose could protect liver from injury induced by CCl4, which may be associated with its antioxidation effect. Key words:Gienoside compound K;Hepatic injury; Alanine aminotraferase; Aspartate aminotraferase; Superoxide dismutase 人参皂苷为人参的主要活性成分，具有抗炎［1］、抗肿瘤［2］等药理活性。这些药理活性已被阐明是人参皂苷在肠道细菌的作用下发生生物转化生成次级代谢产物的生物效应［3～5］。CK是人参皂苷在肠道的主要代谢产物，近年来研究发现其具有抗肿瘤等生物学活性，然而其在肝损伤的保护方面至今未见报道。因此，本实验采用CCl4致大鼠慢性肝损伤模型，观察人参皂苷CK对慢性肝损伤的影响。 1 材料与方法 动物Wistar大鼠，雄性，体重150～180 g，山东省天然药物工程技术研究中心提供，动物合格证号：鲁动质字号。 药品与试剂CK：山东省天然药物工程技术研究中心提供。复方鳖甲软肝片：内蒙古福瑞中蒙药科技股份有限公司生产（批号：）。ALT、AST检测试剂盒：中生北控生物科技股份有限公司生产（批号：010331/020101）；SOD，MDA检测试剂盒：南京建成生物工程研究所生产（批号：/）；HA、PCⅢ酶标检测试剂盒：上海海研医学生物技术中心提供。 造模及用药将60只大鼠随机分为正常对照组、肝损伤模型组、复方鳖甲软肝片 治疗 组、CK小剂量治疗组、CK中剂量治疗组、CK大剂量治疗组，每组10只，共6组。将CCl4与精制花生油按2∶3比例配成40%油剂CCl4，除正常组外其余各组按CCl4 3 ml/kg皮下注射（首次加倍），正常对照组则以同样的方法注射等量的精制花生油，每周2次，持续造模6周，然后灌服相应药物或溶媒，复方鳖甲软肝片组给予复方鳖甲软肝片 g/kg； CK小剂量组给予CK