2300份成年人脑脊液培养及菌株耐药分析.docVIP

2300份成年人脑脊液培养及菌株耐药分析 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：2300份成年人脑脊液培养及菌株耐药分析 1 1 材料与方法 2 2 结果 3 3 讨论 5 文2：颅脑术后细菌性脑膜炎脑脊液培养及药敏结果 6 1 材料与方法 6 2 结果 7 3 讨论 7 参考文摘引言： 8 原创性声明（模板） 9 文章致谢（模板） 10 正文 2300份成年人脑脊液培养及菌株耐药分析 文1：2300份成年人脑脊液培养及菌株耐药分析 Analyses on the pathoge and their resistance to antibiotics ABSTRACT Objective To analyze the pathogenic results and drug resistance of bacteria isolated from 2300 cerebrospinal fluild specime of adults from 2002 to 2005 so as to provide evidence for clinical diagnosis and therapy of meningitis. Methods The bacteria identification and the antimicrobial susceptibility were assayed by automatic bacteria culture device Microscan Walkaway 40 from DADE Co. Results The positive rate of pathoge was % (180/2300) from 2300 cerebrospinal fluild were 144 strai of bacteria which include 98 Gram positive bacteria and 46 Gram negative bacteria, 26 fungi, and 10 Mycobacterium tuberculosis. Streptococcus spp. and Enterococcus spp. were seitive to penicillin, all the Gram positive cocci were seitive to vancomycin; the Gram negative bacteria were resistant to routine antibiotics such as cefotaxime and ceftriaxone, while they have high percentage of susceptibility to aztreonam and carbapenems and some cases were infected with Mycobacterium tuberculosis and fungi. Conclusion It suggests that clinicia should pay more attention to peonalized therapy based on the characteristic of meningitis so as to attain rational use of antibiotics. KEY WORDS Cerebrospinal fluild; Pathogen; Drug resistance 病原菌性脑膜炎通常包括化脓性细菌所引起的急性化脓性脑膜炎以及结核分枝杆菌、深部真菌所引起的慢性脑膜炎，对人类危害极大，位居世界感染性疾病病死率的前十位［1］。病原菌性脑膜炎的诊断依赖脑脊液的检查，脑脊液的细菌培养及药敏结果对临床医生的诊断、用药、疗效判定有着重要的作用［2］。随着社会环境、医疗手段的不断改变，病原菌性脑膜炎的病原体构成、药敏情况值得探讨。本文对四川大学华西 医院 2002年1月至2005年6月2300份脑脊液培养结果分析报道如下。 1 材料与方法 研究对象 四川大学华西医院2002年1月至2005年6月送检的2300份脑脊液标本。 标本采集 以无菌技术采集脑脊液3～5ml，置无菌容器中立即送检。 分离培养 将送检的脑脊液立即接种于已预温的兔血琼脂和不含万古霉素的巧克力平板以及葡萄糖肉汤增菌液中，置5%～10% CO2孵箱，培养24h后观察结果，若肉汤增菌液中呈混浊或有菌膜产生，再挑取培养液或菌膜转种于血平板中，进行细菌分离培养，鉴定步骤按《