芪丹通脉片对急性缺血再灌注大鼠心肌信号转导系统PI3K.docVIP

芪丹通脉片对急性缺血再灌注大鼠心肌信号转导系统PI3K 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：芪丹通脉片对急性缺血再灌注大鼠心肌信号转导系统PI3K 1 1 材料与方法 3 2 结果 5 3 讨论 6 文2：芪丹通脉片对急性缺血再灌注大鼠心肌一氧化氮合酶系统的影响药学 8 1 实验材料 8 2 实验方法 9 3 结果 11 4 讨论 11 参考文摘引言： 13 原创性声明（模板） 15 文章致谢（模板） 15 正文 芪丹通脉片对急性缺血再灌注大鼠心肌信号转导系统PI3K 文1：芪丹通脉片对急性缺血再灌注大鼠心肌信号转导系统PI3K Effect of Qidan Tongmai Tablets on myocardial signal traaction　PI3K/Akt and Erk1/2 in rats with acute ischemia reperfusion ABSTRACT: Objective To investigate the effect of Qidan Tongmai Tablet (QDTMT) on myocardium ischemia reperfusion injury and signal pathway phosphatidylinositol3 kinase (PI3K)/ serinehreonine kinase (Akt) and (the mitogenactivated protein kinase p42/44 extracellular signalregulated kinases (Erk1/2). Methods Male SpragueDawley rats were divided into 3 groups randomly: sham, ischemia reperfusion(model) and QDTMT treatment. The animals were anesthetized and openchest animals were subjected to 40min mycardial ischemia followed by 4h reperfusion after administering saline in sham and model or QDTMT for 7d. The area at risk and infarct size were determined by Evans blue and triphenyl tetrazolium chloride methods; cardiac myocyte apoptosis was determined both qualitatively and quantitatively by terminal deoxynucleotidyl trafeasemediated dUTP by nickend labeling analysis methods. The expression of PI3K signaling cascade and Erk1/2 was determined by Western blotting analysis. Results The IS/AAR and apoptosis index were significantly attenuated respectively in QDTMTtreated animals (±)%,(±)% vs. (±)%, (±)% in models (P, P). The expression of pAkt and pErk1/2 was induced fold and fold higher respectively in QDTMTtreated animals than in models (P, P). Conclusion The results suggest that QDTMT may be able to protect the heart agait reperfusioninduced injury mediated by the increase of phosphorylating Akt and Erk1/2. KEY WORDS: Qidan Tongmai tablet; reperfusion injury; myocardium; phosphatidylinositol3 kinase; serinehreoni