伦尔欣对糖尿病大鼠肾小球血管细胞外基质表达的干预作用.docVIP

伦尔欣对糖尿病大鼠肾小球血管细胞外基质表达的干预作用 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：伦尔欣对糖尿病大鼠肾小球血管细胞外基质表达的干预作用 1 1 材料与方法 3 2 结果 5 3 讨论 6 文2：福辛普利对实验性糖尿病大鼠心肌细胞外基质的影响 8 图1－图3略 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 伦尔欣对糖尿病大鼠肾小球血管细胞外基质表达的干预作用 文1：伦尔欣对糖尿病大鼠肾小球血管细胞外基质表达的干预作用 Intervention of Lunerxin on the expression of glomerulus extracellular　matrix in streptozotocininduced diabetic nephropathy rats ABSTRACT: Objective To observe the regulatory effect of Lunerxin on glomerulus extracellular matrix of streptozotocininduced diabetic rats. Methods Diabetic nephropathy in rats was induced by intraperitoneal injection of streptozotocin. The rats were allocated to normal control group, model group, and Lunerxin group for 16 weeks. After the treatment, blood advanced glycosylation end product level, malonaldehyde (MDA), erythrocuprein (SOD), urinary albumin excretion rate of the 24hou, and clearance rate of creatinine, as well as collagen Ⅰ and HSPG were determined by in situ immunohistochemistry in different groups. Results Diabetes mellitus and renal function lesion occurred in the two model groups. Lunerxin could improve the general state, and decrease advanced glycosylation end products, blood urea nitrogen, serum creatinine, urinary albumin excretion rate of the 24hou and the expression of collagen Ⅰ. It increased clearance rate of creatinine and expression of heparan sulfate proteoglycan in the kidney significantly at the same time. Conclusion Lunerxin has certain protective effect on the kidney by regulating the expression of glomerulus extracellular matrix. KEY WORDS: Lunerxin; diabetic model; extracellular matrix 细胞外基质(extracellular matrix, ECM)散在分布于肾小球，是肾小球基底膜的重要组成成分。Ⅰ型胶原(collagen Ⅰ, CⅠ)与糖尿病肾纤维化关系密切，硫酸乙酰肝素蛋白(heparan sulfate proteoglycan, HSPG)已被证明对保持电荷屏障有重要作用，故两者成为实验研究的重点和热点。目前，糖尿病肾病(diabetic nephropathy, DN)的诊疗进展迅速，糖基化终产物(advanced glycation end products, AGEs)的形成和氧化应激在糖尿病肾病发病中的作用已逐渐被重视[12]，并且在动物实验应用盐酸氨胍特异抑制AGEs的形成，延缓糖尿病肾病的发生、 发展 起到一定的效果。但是，由于其毒副作用大